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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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Blk Monoclonal Antibody
- 货号:YM0067
- 应用:WB;IF;FCM;ELISA
- 种属:Human
- 蛋白名称:
- Tyrosine-protein kinase Blk
- 免疫原:
- Purified recombinant fragment of human Blk expressed in E. Coli.
- 特异性:
- Blk Monoclonal Antibody detects endogenous levels of Blk protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释:
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- Affinity purification
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- BLK;Tyrosine-protein kinase Blk;B lymphocyte kinase;p55-Blk
- 背景:
- This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010],
- 功能:
- catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:May function in a signal transduction pathway that is restricted to B-lymphoid cells.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,
- 细胞定位:
- Cell membrane ; Lipid-anchor . Present and active in lipid rafts. Membrane location is required for the phosphorylation of CD79A and CD79B (By similarity). .
- 组织表达:
- Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles.
![](/uploadfiles/PIC/ym0067-wb.jpg)
- Western Blot analysis using Blk Monoclonal Antibody against HEK293 (1) and BLK-hIgGFc transfected HEK293 (2) cell lysate.
![](/uploadfiles/PIC/ym0067-if.jpg)
- Immunofluorescence analysis of Hela cells using Blk Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
![](/uploadfiles/PIC/ym0067-fcm.jpg)
- Flow cytometric analysis of HL-60 cells using Blk Monoclonal Antibody (green) and negative control (purple).