ALDH1A1 Monoclonal Antibody
- 货号：YM0022
- 应用：WB;IHC;IF;ELISA
- 种属：Human
- 简介：
- >>Retinol metabolism;>>Metabolic pathways
- 蛋白名称：
- Retinal dehydrogenase 1
- 免疫原：
- Purified recombinant fragment of human ALDH1A1 expressed in E. Coli.
- 特异性：
- ALDH1A1 Monoclonal Antibody detects endogenous levels of ALDH1A1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ALDH1A1;ALDC;ALDH1;PUMB1;Retinal dehydrogenase 1;RALDH 1;RalDH1;ALDH-E1;ALHDII;Aldehyde dehydrogenase family 1 member A1;Aldehyde dehydrogenase; cytosolic
- 背景：
- The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011],
- 功能：
- catalytic activity:Retinal + NAD(+) + H(2)O = retinoate + NADH.,function:Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.,pathway:Cofactor metabolism; retinol metabolism.,similarity:Belongs to the aldehyde dehydrogenase family.,subunit:Homotetramer.,
- 细胞定位：
- Cytoplasm, cytosol . Cell projection, axon .
- 组织表达：
- Expressed by erythrocytes (at protein level).
- Western Blot analysis using ALDH1A1 Monoclonal Antibody against Raji (1), Jurkat (2), THP-1 (3) and K562 (4) cell lysate.
- Immunohistochemistry analysis of paraffin-embedded cerebellum tissues (left) and lung cancer (right) with DAB staining using ALDH1A1 Monoclonal Antibody.
