4E-BP1 Monoclonal Antibody
- 货号：YM0002
- 应用：WB;IHC;IF;ELISA
- 种属：Human
- 简介：
- >>EGFR tyrosine kinase inhibitor resistance;>>ErbB signaling pathway;>>HIF-1 signaling pathway;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Cellular senescence;>>Insulin signaling pathway;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Herpes simplex virus 1 infection;>>Chemical carcinogenesis - receptor activation;>>Acute myeloid leukemia;>>Choline metabolism in cancer
- 蛋白名称：
- Eukaryotic translation initiation factor 4E-binding protein 1
- 免疫原：
- Purified recombinant fragment of 4E-BP1 expressed in E. Coli.
- 特异性：
- 4E-BP1 Monoclonal Antibody detects endogenous levels of 4E-BP1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- EIF4EBP1;Eukaryotic translation initiation factor 4E-binding protein 1;4E-BP1;eIF4E-binding protein 1;Phosphorylated heat- and acid-stable protein regulated by insulin 1;PHAS-I
- 背景：
- eukaryotic translation initiation factor 4E binding protein 1(EIF4EBP1) Homo sapiens This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008],
- 功能：
- function:Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway.,PTM:Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the eIF4E-binding protein family.,subunit:Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.,
- 细胞定位：
- nucleoplasm,cytoplasm,cytosol,protein complex,
- 组织表达：
- Colon,Epithelium,Lung,Placenta,Platelet,
- Western Blot analysis using 4E-BP1 Monoclonal Antibody against truncated 4E-BP1 recombinant protein (1) and A431 cell lysate (2).
- Immunohistochemistry analysis of paraffin-embedded human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue (C), showing cytoplasmic and membrane localization with DAB staining using 4E-BP1 Monoclonal Antibody.
