Histone H2A.Z (Acetyl Lys7) Polyclonal Antibody
- 货号：YK0203
- 应用：WB
- 种属：Human;Mouse;Rat
- 简介：
- >>Necroptosis;>>Neutrophil extracellular trap formation;>>Alcoholism;>>Systemic lupus erythematosus
- 免疫原：
- Synthetic Peptide of Histone H2A.Z (Acetyl Lys7)
- 特异性：
- The antibody detects endogenous Histone H2A.Z (Acetyl Lys7) protein.
- 组成：
- PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
- 来源：
- Polyclonal, Rabbit,IgG
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- H2AFZ;H2AZ;Histone H2A.Z;H2A/z
- 背景：
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008],
- 功能：
- function:Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.,mass spectrometry:Monoisotopic, not modified PubMed:16457589,PTM:Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.,PTM:Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.,PTM:Not phosphor
- 细胞定位：
- Nucleus. Chromosome.
- 组织表达：
- Brain,Epithelium,Skeletal muscle,Uterus,
- Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
