EKLF (Acetyl Lys274) rabbit pAb
- 货号：YK0151
- 应用：WB;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- EKLF (Acetyl Lys274)
- 免疫原：
- Synthesized peptide derived from human EKLF (Acetyl Lys274)
- 特异性：
- This antibody detects endogenous levels of Human EKLF (Acetyl Lys274)
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:1000-2000 ELISA 1:5000-20000
- 纯化工艺：
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- Krueppel-like factor 1 (Erythroid krueppel-like transcription factor;EKLF)
- 背景：
- This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009],
- 功能：
- function:Transcription regulator of erythrocyte development. Binds to the CACCC box in the beta-globin gene promoter and activates transcription. When sumoylation, acts as a Probably serves as a general switch factor for erythroid development. When sumoylated, acts as a transcriptional repressor, by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.,PTM:Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.,PTM:Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.,PTM:Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.,simila
- 细胞定位：
- Nucleus . Colocalizes with SUMO1 in nuclear speckles. .
- 组织表达：
- Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.
