Recombinant Human VEGF165 protein ,C- His Tag
- 纯度：
- >90% as determined by SDS-PAGE
- 体内研究：
- Please contact with the lab for this information.
- 序列：
- A DNA sequence encoding the human VEGFA(Met1-Arg191) was fused the C-terminal His Tag
- 组成：
- Supplied as solution form in PBS pH 7.5./ Lyophilized from PBS pH 7.5.
- 稀释：
- Reconstitute in sterile water for a stock solution
- 浓度：
- >90% as determined by SDS-PAGE
- 储存：
- Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.
- 细胞定位：
- Secreted . VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a significant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
- 组织表达：
- Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland.
