Recombinant Human EGFR protein ,C- His tag
- 纯度：
- >90% as determined by SDS-PAGE
- 体内研究：
- < 1.0 EU per μg of the protein as determined by the LAL method.
- 序列：
- A DNA sequence encoding the human EGFR(Met1-Ser645) was fused with the C-terminal His tag
- 组成：
- Supplied as solution form in PBS pH 7.5./ Lyophilized from PBS pH 7.5.
- 稀释：
- Reconstitute in sterile water for a stock solution
- 浓度：
- >90% as determined by SDS-PAGE
- 储存：
- Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.
- 细胞定位：
- Cell membrane ; Single-pass type I membrane protein . Endoplasmic reticulum membrane ; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome . Endosome membrane. Nucleus . In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER (PubMed:20674546, PubMed:17909029). Endocytosed upon activation by ligand (PubMed:2790960, PubMed:17182860, PubMed:27153536, PubMed:17909029). Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF) (PubMed:20551055). .; [Isoform 2]: Secreted.
- 组织表达：
- Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
