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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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S100 protein
- 货号:YD0088
- 应用:WB;SDS-PAGE
- 种属:Human
- 序列:
- Amino acid: full length, with his-MBP tag.
- 储存:
- -20°C/6 month,-80°C for long storage
- 其他名称:
- Protein S100-A1 (S-100 protein alpha chain) (S-100 protein subunit alpha) (S100 calcium-binding protein A1)
- 背景:
- function:Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.,similarity:Belongs to the S-100 family.,similarity:Contains 2 EF-hand domains.,subunit:Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain.,tissue specificity:Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.,
- 功能:
- negative regulation of transcription from RNA polymerase II promoter, regulation of transcription, DNA-dependent,regulation of transcription from RNA polymerase II promoter, intracellular signaling cascade, regulation of heart contraction, negative regulation of biosynthetic process, negative regulation of macromolecule biosynthetic process,negative regulation of macromolecule metabolic process, negative regulation of gene expression, negative regulation of transcription, negative regulation of cellular biosynthetic process, regulation of system process, regulation of transcription, negative regulation of transcription, DNA-dependent, negative regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolic process, negative regulation of nitrogen compound metabolic process, regulation of RNA metabolic process, negative regulation of RNA metabolic process,
- 细胞定位:
- Cytoplasm . Sarcoplasmic reticulum . Mitochondrion .
- 组织表达:
- Highly prevalent in heart (PubMed:12804600, PubMed:1384693). Also found in lesser quantities in skeletal muscle and brain (PubMed:1384693).