ImmunoWay Biotechnology Company |抗体|病理抗体|诊断抗体

Phospho PKC δ (Y64) Cell-Based Colorimetric ELISA Kit

其他名称：
Protein kinase C delta type (EC 2.7.11.13) (Tyrosine-protein kinase PRKCD) (EC 2.7.10.2) (nPKC-delta)

背景：
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.,domain:The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner.,enzyme regulation:Three specific sites; Thr-507 (activation loop of the kinase domain), Ser-645 (turn motif) and Ser-664 (hydrophobic region), need to be phosphorylated for its full activation.,function:This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.,PTM:Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 C2 domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Interacts with PDK1, RAD9A, CDCP1 and MUC1.,

功能：
cell activation, cytokine production, regulation of protein amino acid phosphorylation, negative regulation of protein amino acid phosphorylation, adaptive immune response, immune effector process, leukocyte mediated immunity,lymphocyte mediated immunity, adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains, protein amino acid phosphorylation, phosphorus metabolic process,phosphate metabolic process, immune response, intracellular signaling cascade, aging, cell proliferation, negative regulation of signal transduction, senescence, regulation of receptor activity, negative regulation of phosphorus metabolic process, negative regulation of macromolecule metabolic process, posttranscriptional regulation of gene expression, negative regulation of cell communication, immunoglobulin mediated immune response, phosphorylation,r

细胞定位：
Cytoplasm . Cytoplasm, perinuclear region . Nucleus . Cell membrane ; Peripheral membrane protein . Mitochondrion . Endomembrane system . Translocates to the mitochondria upon apoptotic stimulation. Upon activation, translocates to the plasma membrane followed by partial location to the endolysosomes (PubMed:17303575). .