Ki-67 Rabbit mAb(ABT104R)
- 货号：IHCM7002
- 应用：IHC
- 种属：Human
- 免疫原：
- Synthesized peptide derived from human Ki-67
- 特异性：
- The antibody can specifically recognize human Ki-67 protein.
- 来源：
- Monoclonal, Rabbit,IgG
- 纯化工艺：
- The antibody was affinity-purified from Cell supernatant by affinity-chromatography using specific immunogen.
- 其他名称：
- MKI67;Antigen KI-67
- 背景：
- This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009],
- 功能：
- developmental stage:Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.,function:Thought to be required for maintaining cell proliferation.,online information:Ki-67 entry,similarity:Contains 1 FHA domain.,subcellular location:Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.,subunit:Interacts with KIF15. Binds through the FHA domain to MKI67IP.,
- Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section).
The immunostaining was performed by Pentuple-Fluorescence kit (RS0038, Immunoway). CK-pan mouse mAb(YM6815 Immunoway) green, Ki-67 rabbit mAb(YM7002 Immunoway) red, Iba 1 mouse mAb(YM4765
Immunoway) purple,CD8 a mouse mAb(YM4815
Immunoway) cyan, CD20 mouse mAb(YM4814 Immunoway) yellow,
The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Immunoway YS0004, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).
