Multi Fluorescence Immunohistochemistry/Cytochemical Detection Kit, abbreviated as Multi-color reagent kit. This kit contains relevant reagents required for mIHC/mIF experiments, including dewaxing repair two in one solution, peroxidase blocking buffer, polymer enzyme-linked immunosorbent assay (ELISA) secondary antibody, fluorescently labeled Tyramide, Tyramide diluent, anti quenched DAPI sealing two in one solution, and antibody stripping solution.

Detection principle: Based on Tyramide signal amplification (TSA) technology, multi index analysis of the same slice is achieved, presenting a panoramic analysis from point to surface. In situ display and quantitative analysis; Display spatial relationships and differentiate cell subgroups; Simultaneous analysis of target and microenvironment; Multi target free combination, not limited by the source of primary antibody species.

Dyeing effect diagram:

Mouse Rabbit Three Standard Four Color Fluorescence Detection Kit (RS0035)

Fluorescence multiplex immunohistochemical analysis of normal human appendix tissue (formalin-fixed paraffin-embedded section).The section was incubated in 3 rounds of staining; in the order of CK PAN .( Catalog no:YM6815 1/200 dilution), PD-1.(Catalog no: YM6208 1/200 dilution), Caldesmon pan. (Catalog no:YM6826 1/200 dilution),each using a separate fluorescent tyramide signal amplification system : Treble-Fluorescence immunohistochemical mouse/rabbit kit Catalog NO: RS0035 (pH9.0)

Mouse Rabbit Five Standard Six Color Fluorescence Detection Kit (RS0038)

Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Pentuple-Fluorescence kit (RS0038, Immunoway). CK-pan mouse mAb(YM6815 Immunoway) green, Ki-67 rabbit mAb(YM7002 Immunoway) red, Iba 1 mouse mAb(YM4765 Immunoway) purple,CD8 a mouse mAb(YM4815 Immunoway) cyan, CD20 mouse mAb(YM4814 Immunoway) yellow, The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Immunoway YS0004, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).

Mouse Rabbit Six Standard Seven Color Fluorescence Detection Kit (RS0039)

Fluorescence multiplex immunohistochemical analysis of Mouse brain tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Sextuple-Fluorescence kit (RS0039, Immunoway). GFAP mouse mAb(YM4426 Immunoway) green, S100 mouse mAb(YM6987 Immunoway) cyan,Neurofilament mouse mAb(YM6897 Immunoway) purple,Iba 1 mouse mAb(YM4765 Immunoway) red,CD8 a mouse mAb(YM4815 Immunoway) yellow, The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Immunoway YS0004, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).

List of multi-color reagent kit products

Note: The mild multi-color kit does not require heating to boiling during antibody stripping, and can be reacted at 37 ℃.