PPP2CA

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform ;
PP2A-alpha ;
Replication protein C ;
RP-C ;

catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,cofactor:Binds 1 iron ion per subunit.,cofactor:Binds 1 manganese ion per subunit.,function:PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.,PTM:Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.,PTM:Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle.,similarity:Belongs to the PPP phosphatase family.,similarity:Belongs to the PPP phosphatase family. PP-1 subfamily.,subcellular location:In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.,subunit:PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with NXN; the interaction is direct (By similarity). May indirectly interact with SGOL1, most probably through regulatory B56 subunits.,

MAPKKK cascade, inactivation of MAPK activity, regulation of cell growth, regulation of protein amino acid phosphorylation, negative regulation of protein amino acid phosphorylation, regulation of DNA replication, RNA processing, negative regulation of protein kinase activity, protein amino acid dephosphorylation, membrane lipid metabolic process, sphingolipid metabolic process, ceramide metabolic process, phosphorus metabolic process,phosphate metabolic process, induction of apoptosis, intracellular signaling cascade, protein kinase cascade,mesoderm development, regulation of cell size, RNA splicing, negative regulation of signal transduction, response to organic substance, negative regulation of phosphorus metabolic process, negative regulation of macromolecule metabolic process, regulation of protein kinase cascade, negative regulation of cell communication, negative regulation of protein kinase cascade, regulation of cell death, positive regulation of cell death, induction of programmed cell death, dephosphorylation, regulation of phosphate metabolic process, second-messenger-mediated signaling, regulation of Wnt receptor signaling pathway, regulation of cell adhesion, negative regulation of cell growth,regulation of protein modification process, negative regulation of protein modification process, regulation of cellular protein metabolic process, negative regulation of cellular protein metabolic process, regulation of cellular component size, negative regulation of kinase activity, regulation of growth, regulation of phosphorylation, negative regulation of phosphorylation, regulation of tyrosine phosphorylation of STAT protein, regulation of tyrosine phosphorylation of Stat3 protein, negative regulation of tyrosine phosphorylation of Stat3 protein, negative regulation of tyrosine phosphorylation of STAT protein, regulation of apoptosis, positive regulation of apoptosis, regulation of programmed cell death, positive regulation of programmed cell death, negative regulation of catalytic activity, regulation of MAP kinase activity, negative regulation of MAP kinase activity, regulation of kinase activity, negative regulation of molecular function, regulation of transcription, negative regulation of cell size, regulation of protein kinase activity,negative regulation of growth, negative regulation of phosphate metabolic process, regulation of JAK-STAT cascade,negative regulation of JAK-STAT cascade, sphingoid metabolic process, regulation of peptidyl-tyrosine phosphorylation,negative regulation of peptidyl-tyrosine phosphorylation, regulation of DNA metabolic process, regulation of phosphorus metabolic process, negative regulation of protein metabolic process, regulation of transferase activity,negative regulation of transferase activity,

Cytoplasm . Nucleus . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle pole . In prometaphase cells, but not in anaphase cells, localizes at centromeres (PubMed:16541025). During mitosis, also found at spindle poles (PubMed:16541025). Centromeric localization requires the presence of SGO2 (By similarity). .