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Main Information
Target

V5-Tag

Host Species

Mouse

Reactivity

Species independent

Applications

WB, IP, IF

Conjugate/Modification


Unmodified

Detailed Information
Recommended Dilution Ratio
WB 1:5000; IP 1:200; IF 1:1000
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Specificity
The antibody detects C-terminal, internal, and N-terminal V5-tag fusion proteins.
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Modification
Unmodified
Clonality
Monoclonal
Clone Number
3C8
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Antigen&Target Information
Immunogen:
Synthetic Peptide of V5-Tag
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Specificity:
The antibody detects C-terminal, internal, and N-terminal V5-tag fusion proteins.
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Background:
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein. This V5-tag antibody is prepared by immunizing synthetic peptide GKPIPNPLLGLDST coupled to KLH.
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