Optimal working dilutions should be determined experimentally by the investigator; Suggested starting dilutions are as follows:WB 1:3000-10000.
Formulation
Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.
Specificity
His-Tag Monoclonal Antibody(4E6) specially designed for your taged protein quantification and localization.
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage
Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Concentration
1mg/ml
Conjugate
HRP
Clonality
Monoclonal
Clone Number
4E6
Isotype
IgG
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Antigen&Target Information
Specificity:
His-Tag Monoclonal Antibody(4E6) specially designed for your taged protein quantification and localization.
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Gene Name:
His-Tag
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Protein Name:
His Tag
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Other Name:
6 His epitope tag ;
Hexa His tag ;
HHHHHH epitope tag ;
HHHHHH tag ;
His tag
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Background:
The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.