• Target：
• EKLF

• Gene Name：
• KLF1

• Protein Name：
• Krueppel-like factor 1

• Human Gene Id：
• 10661

• Human Swiss Prot No：
• Q13351

• Mouse Swiss Prot No：
• P46099

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human KLF1 around the non-acetylation site of Lys274. AA range:231-280

• Specificity：
• EKLF Polyclonal Antibody detects endogenous levels of EKLF protein only when acetylation at K274.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• KLF1;EKLF;Krueppel-like factor 1;Erythroid krueppel-like transcription factor;EKLF

• Observed Band(KD)：
• 38kD

• Background：
• This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009],

• Function：
• function:Transcription regulator of erythrocyte development. Binds to the CACCC box in the beta-globin gene promoter and activates transcription. When sumoylation, acts as a Probably serves as a general switch factor for erythroid development. When sumoylated, acts as a transcriptional repressor, by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.,PTM:Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.,PTM:Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.,PTM:Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.,simila

• Subcellular Location：
• Nucleus . Colocalizes with SUMO1 in nuclear speckles. .

• Expression：
• Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.

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