• Target：
• SIGLEC8

• Gene Name：
• SIGLEC8

• Protein Name：
• Sialic acid-binding Ig-like lectin 8

• Human Gene Id：
• 27181

• Human Swiss Prot No：
• Q9NYZ4

• Immunogen：
• The antiserum was produced against synthesized peptide derived from the Internal region of human SIGLEC8. AA range:81-130

• Specificity：
• CD329 Polyclonal Antibody detects endogenous levels of CD329 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• SIGLEC8;SAF2;Sialic acid-binding Ig-like lectin 8;Siglec-8;CDw329;Sialoadhesin family member 2;SAF-2;CD329

• Observed Band(KD)：
• 50kD

• Background：
• Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010],

• Function：
• domain:Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.,function:Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.,online information:Siglec-8,similarity:Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,similarity:Contains 2 Ig-like C2-type (immunoglobulin-like) domains.,tissue specificity:Expressed specifically on eosin

• Subcellular Location：
• Membrane; Single-pass type I membrane protein.

• Expression：
• Expressed specifically on red blood cells namely basophil, mast cells and eosinophils.

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