• Target：
• Cdc34

• Fields：
• >>Ubiquitin mediated proteolysis

• Gene Name：
• CDC34

• Protein Name：
• Ubiquitin-conjugating enzyme E2 R1

• Human Gene Id：
• 997

• Human Swiss Prot No：
• P49427

• Mouse Gene Id：
• 216150

• Mouse Swiss Prot No：
• Q8CFI2

• Immunogen：
• The antiserum was produced against synthesized peptide derived from the Internal region of human CDC34. AA range:121-170

• Specificity：
• Cdc34 Polyclonal Antibody detects endogenous levels of Cdc34 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• CDC34;UBCH3;UBE2R1;Ubiquitin-conjugating enzyme E2 R1;Ubiquitin-conjugating enzyme E2-32 kDa complementing;Ubiquitin-conjugating enzyme E2-CDC34;Ubiquitin-protein ligase R1

• Observed Band(KD)：
• 34kD

• Background：
• The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.,function:Catalyzes the covalent attachment of ubiquitin to other proteins. May be involved in degradation of katenin.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the ubiquitin-conjugating enzyme family.,subunit:Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. When phosphorylated, interacts with beta-TrCP (BTRC).,

• Subcellular Location：
• Cytoplasm. Nucleus. The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.

• Expression：
• Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.

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