• Target：
• V-ATPase H

• Fields：
• >>Oxidative phosphorylation;>>Metabolic pathways;>>Lysosome;>>Phagosome;>>mTOR signaling pathway;>>Synaptic vesicle cycle;>>Vibrio cholerae infection;>>Epithelial cell signaling in Helicobacter pylori infection;>>Tuberculosis;>>Human papillomavirus infection;>>Rheumatoid arthritis

• Gene Name：
• ATP6V1H

• Protein Name：
• V-type proton ATPase subunit H

• Human Gene Id：
• 51606

• Human Swiss Prot No：
• Q9UI12

• Mouse Gene Id：
• 108664

• Mouse Swiss Prot No：
• Q8BVE3

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human ATP6V1H. AA range:341-390

• Specificity：
• V-ATPase H Polyclonal Antibody detects endogenous levels of V-ATPase H protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ATP6V1H;CGI-11;V-type proton ATPase subunit H;V-ATPase subunit H;Nef-binding protein 1;NBP1;Protein VMA13 homolog;V-ATPase 50/57 kDa subunits;Vacuolar proton pump subunit H;Vacuolar proton pump subunit SFD

• Observed Band(KD)：
• 55kD

• Background：
• This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles. V-ATPase-dependent organelle acidification is necessary for multiple processes including protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The encoded protein is the regulatory H subunit of the V1 domain of V-ATPase, which is required for catalysis of ATP but not the assembly of V-ATPase. Decreased expression of this gene may play a role in the development of type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],

• Function：
• function:Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes.,similarity:Belongs to the V-ATPase H subunit family.,subunit:V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). Interacts with HIV-1 Nef protein and AP2M1.,tissue specificity:Widely expressed.,

• Subcellular Location：
• Cytoplasmic vesicle, clathrin-coated vesicle membrane ; Peripheral membrane protein .

• Expression：
• Widely expressed.

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