TLK1 Polyclonal Antibody

    • Catalog No.:YT4673
    • Applications:IF,ELISA
    • Reactivity:Human,Mouse
      • Gene Name:
      • TLK1
      • Protein Name:
      • Serine/threonine-protein kinase tousled-like 1
      • Human Gene Id:
      • 9874
      • Human Swiss Prot No:
      • Q9Y4F6
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human TLK1. AA range:730-779
      • Specificity:
      • TLK1 Polyclonal Antibody detects endogenous levels of TLK1 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • TLK1; KIAA0137; Serine/threonine-protein kinase tousled-like 1; PKU-beta; Tousled-like kinase 1
      • MolecularWeight(Da):
      • 86700
      • Background:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Cell-cycle regulated, maximal activity in S-phase. Inactivated by phosphorylation at Ser-743, potentially by CHK1.,function:Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by faciliting the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Heterodimerizes with TLK2. Interacts with ASF1A and ASF1B.,tissue specificity:Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.,
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Cell-cycle regulated, maximal activity in S-phase. Inactivated by phosphorylation at Ser-743, potentially by CHK1.,function:Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by faciliting the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase d
      • Products Images
      • Immunofluorescence analysis of COS7 cells, using TLK1 Antibody. The picture on the right is blocked with the synthesized peptide.