• Target：
• Netrin-1

• Fields：
• >>Axon guidance

• Gene Name：
• NTN1

• Protein Name：
• Netrin-1

• Human Gene Id：
• 9423

• Human Swiss Prot No：
• O95631

• Mouse Gene Id：
• 18208

• Mouse Swiss Prot No：
• O09118

• Rat Gene Id：
• 114523

• Rat Swiss Prot No：
• Q924Z9

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human Netrin-1. AA range:484-533

• Specificity：
• Netrin-1 Polyclonal Antibody detects endogenous levels of Netrin-1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NTN1;NTN1L;Netrin-1;Epididymis tissue protein Li 131P

• Observed Band(KD)：
• 75kD

• Background：
• Netrin is included in a family of laminin-related secreted proteins. The function of this gene has not yet been defined; however, netrin is thought to be involved in axon guidance and cell migration during development. Mutations and loss of expression of netrin suggest that variation in netrin may be involved in cancer development. [provided by RefSeq, Jul 2008],

• Function：
• disease:Defects in NTN1 are associated with some forms of neuroblastomas.,function:Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis.,similarity:Contains 1 laminin N-terminal domain.,similarity:Contains 1 NTR domain.,similarity:Contains 3 laminin EGF-like domains.,subunit:Binds to its receptors; DCC, UNC5A, UNC5B, UNC5C and probably UNC5D.,tissue specificity:Widely expressed in normal adult tissues with highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas.,

• Subcellular Location：
• Secreted . Cytoplasm . Mainly secreted. .

• Expression：
• Widely expressed in normal adult tissues with highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas. Expressed in epididymis (at protein level).

Guo, Xiao-Kai, et al. "The expression of netrin-1 in the thymus and its effects on thymocyte adhesion and migration." Clinical and Developmental Immunology 2013 (2013).

Regulation of Axon Guidance by Slit2 and Netrin-1 Signaling in the Lacrimal Gland of Aqp5 Knockout Mice. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE Peng Chen IF Mouse lacrimal gland (LG) tissue lacrimal gland (LG) cell

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