NEEP21 Polyclonal Antibody
- Catalog No.:YT3028
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- NEEP21
- Gene Name:
- NSG1
- Protein Name:
- Neuron-specific protein family member 1
- Human Gene Id:
- 27065
- Human Swiss Prot No:
- P42857
- Mouse Gene Id:
- 18196
- Mouse Swiss Prot No:
- Q62092
- Rat Gene Id:
- 25247
- Rat Swiss Prot No:
- P02683
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human NSG1. AA range:112-161
- Specificity:
- NEEP21 Polyclonal Antibody detects endogenous levels of NEEP21 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- NSG1;D4S234;Neuron-specific protein family member 1;Brain neuron cytoplasmic protein 1
- Observed Band(KD):
- 26kD
- Background:
- similarity:Belongs to the NSG family.,
- Function:
- similarity:Belongs to the NSG family.,
- Subcellular Location:
- Membrane ; Single-pass type II membrane protein . Golgi apparatus, trans-Golgi network membrane . Endosome membrane . Cell projection, dendrite . Early endosome membrane . Late endosome membrane . Lysosome lumen . Recycling endosome membrane . Cytoplasmic vesicle membrane . Golgi apparatus, Golgi stack membrane . Endosome, multivesicular body membrane . Endoplasmic reticulum membrane . Endocytosed from the cell surface, thus enters into early endosomes, trafficks to late endosomes and degradates in lysosomes (By similarity). Endoplasmic reticulum targeting is essential for apoptosis (PubMed:20599942). Found in both stationary and motile endosomes. A previous study supports a type I membrane protein topology (By similarity). .
- Expression:
- Amygdala,Brain,Cerebellum,Testis,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using NEEP21 Polyclonal Antibody diluted at 1:2000
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from COLO cells, using NSG1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from K562 cells using NSG1 antibody.