EGFR Polyclonal Antibody

    • Catalog No.:YT1493
    • Applications:WB,ELISA
    • Reactivity:Human,Mouse
    • Description
    • Gene Name:
    • EGFR
    • Protein Name:
    • Epidermal growth factor receptor
    • Human Gene Id:
    • 1956
    • Human Swiss Prot No:
    • P00533
    • Mouse Swiss Prot No:
    • Q01279
    • Immunogen:
    • The antiserum was produced against synthesized peptide derived from human EGFR. AA range:1077-1126
    • Specificity:
    • EGFR Polyclonal Antibody detects endogenous levels of EGFR protein.
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Rabbit
    • Dilution:
    • Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
    • Purification:
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
    • MolecularWeight(Da):
    • 134277
    • Observed Band(KD):
    • 175
    • Background:
    • Epidermal growth factor receptor encoded by EGFR is a transmembrane glycoprotein that is a member of the protein kinase superfamily. Epidermal growth factor receptor is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of tepidermal growth factor receptor to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in EGFR are associated with lung cancer.
    • Function:
    • regulation of cyclin-dependent protein kinase activity, G1/S transition of mitotic cell cycle, MAPKKK cascade, activation of MAPKK activity, mitotic cell cycle, cell morphogenesis, skeletal system development, ossification, cell activation,regulation of protein amino acid phosphorylation, translation, protein amino acid phosphorylation, phosphorus metabolic process, phosphate metabolic process, response to osmotic stress, response to oxidative stress, cell cycle,cell adhesion, cell surface receptor linked signal transduction, enzyme linked receptor protein signaling pathway,transmembrane receptor protein tyrosine kinase signaling pathway, epidermal growth factor receptor signaling pathway, activation of phospholipase C activity, intracellular signaling cascade, protein kinase cascade, regulation of mitotic cell cycle, sensory organ development, salivary gland development, salivary gland
    • Products Images
    • Western Blot analysis of various cells using EGFR Polyclonal Antibody
    • Western blot analysis of lysates from A431 cells, treated with EGF 200ng/ml 10', using EGFR Antibody. The lane on the right is blocked with the synthesized peptide.