EGFR Polyclonal Antibody

    • Catalog No.:YT1493
    • Applications:WB,ELISA
    • Reactivity:Human,Mouse
      • Gene Name:
      • EGFR
      • Protein Name:
      • Epidermal growth factor receptor
      • Human Gene Id:
      • 1956
      • Human Swiss Prot No:
      • P00533
      • Mouse Swiss Prot No:
      • Q01279
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human EGFR. AA range:1077-1126
      • Specificity:
      • EGFR Polyclonal Antibody detects endogenous levels of EGFR protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
      • MolecularWeight(Da):
      • 134277
      • Observed Band(KD):
      • 175
      • Background:
      • epidermal growth factor receptor(EGFR) Homo sapiens The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016],
      • Function:
      • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Defects in EGFR are associated with lung cancer [MIM:211980].,function:Isoform 2/truncated isoform may act as an antagonist.,function:Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin.,miscellaneous:Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.,online information:EGFR entry,PTM:Monoubiquitinated and polyubiquitinated upon EGF stimu
      • Subcellular Location:
      • Golgi membrane,extracellular space,nucleus,cytoplasm,endosome,endoplasmic reticulum membrane,plasma membrane,cell-cell adherens junction,focal adhesion,cell surface,endosome membrane,membrane,integral componen
      • Expression:
      • Brain,Epithelium,Hepatocyte,Liver,PCR rescued clones,Placenta,
      • Products Images
      • Western Blot analysis of various cells using EGFR Polyclonal Antibody
      • Western blot analysis of lysates from A431 cells, treated with EGF 200ng/ml 10', using EGFR Antibody. The lane on the right is blocked with the synthesized peptide.