c-Myc Polyclonal Antibody

    • Catalog No.:YT0994
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • MYC
      • Protein Name:
      • Myc proto-oncogene protein
      • Human Gene Id:
      • 4609
      • Human Swiss Prot No:
      • P01106
      • Mouse Swiss Prot No:
      • P01108
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human MYC. AA range:340-389
      • Specificity:
      • c-Myc Polyclonal Antibody detects endogenous levels of c-Myc protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • MYC; BHLHE39; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64
      • MolecularWeight(Da):
      • 48804
      • Observed Band(KD):
      • 50,(also ~60KD in some samples)
      • Background:
      • v-myc avian myelocytomatosis viral oncogene homolog(MYC) Homo sapiens The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008],
      • Function:
      • disease:A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.,disease:Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.,function:Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.,online information:Myc entry,PTM:Phosphorylated by PRKDC.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with KDM5A and KDM5B.,
      • Subcellular Location:
      • nucleus,nucleoplasm,nucleolus,mitochondrion,cytosol,protein complex,
      • Expression:
      • Cervix,Epithelium,Leukemia,Placenta,Promyelocytic l
      • Products Images
      • Western Blot analysis of Jurkat cells using c-Myc Polyclonal Antibody
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MYC Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from Jurkat/293, using MYC Antibody. The lane on the right is blocked with the synthesized peptide.