c-Myc Polyclonal Antibody

    • Catalog No.:YT0994
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human,Mouse,Rat
    • Description
    • Gene Name:
    • MYC
    • Protein Name:
    • Myc proto-oncogene protein
    • Human Gene Id:
    • 4609
    • Human Swiss Prot No:
    • P01106
    • Mouse Swiss Prot No:
    • P01108
    • Immunogen:
    • The antiserum was produced against synthesized peptide derived from human MYC. AA range:340-389
    • Specificity:
    • c-Myc Polyclonal Antibody detects endogenous levels of c-Myc protein.
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Rabbit
    • Dilution:
    • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
    • Purification:
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • MYC; BHLHE39; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64
    • MolecularWeight(Da):
    • 48804
    • Observed Band(KD):
    • 50,(also ~60KD in some samples)
    • Background:
    • The protein encoded by MYC is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
    • Function:
    • DNA catabolic process, endonucleolytic, skeletal system development, B cell apoptosis, release of cy
    • Products Images
    • Western Blot analysis of Jurkat cells using c-Myc Polyclonal Antibody
    • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MYC Antibody. The picture on the right is blocked with the synthesized peptide.
    • Western blot analysis of lysates from Jurkat/293, using MYC Antibody. The lane on the right is blocked with the synthesized peptide.