• Target：
• c-Fos

• Fields：
• >>Endocrine resistance;>>MAPK signaling pathway;>>cAMP signaling pathway;>>Apoptosis;>>Osteoclast differentiation;>>Toll-like receptor signaling pathway;>>IL-17 signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>TNF signaling pathway;>>Circadian entrainment;>>Cholinergic synapse;>>Dopaminergic synapse;>>Estrogen signaling pathway;>>Prolactin signaling pathway;>>Oxytocin signaling pathway;>>Relaxin signaling pathway;>>Parathyroid hormone synthesis, secretion and action;>>Non-alcoholic fatty liver disease;>>Growth hormone synthesis, secretion and action;>>Amphetamine addiction;>>Pathogenic Escherichia coli infection;>>Salmonella infection;>>Pertussis;>>Yersinia infection;>>Leishmaniasis;>>Chagas disease;>>Hepatitis B;>>Measles;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Human immunodeficiency virus 1 infection;>>Coronavirus disease - CO

• Gene Name：
• FOS

• Protein Name：
• Proto-oncogene c-Fos

• Human Gene Id：
• 2353

• Human Swiss Prot No：
• P01100

• Mouse Gene Id：
• 14281

• Mouse Swiss Prot No：
• P01101

• Rat Gene Id：
• 140675

• Rat Swiss Prot No：
• P12841

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human Fos. AA range:1-50

• Specificity：
• c-Fos Polyclonal Antibody detects endogenous levels of c-Fos protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• FOS;G0S7;Proto-oncogene c-Fos;Cellular oncogene fos;G0/G1 switch regulatory protein 7

• Observed Band(KD)：
• 62kD

• Background：
• The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008],

• Function：
• function:Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, c-fos and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.,PTM:Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.,PTM:Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK

• Subcellular Location：
• Nucleus. Endoplasmic reticulum. Cytoplasm, cytosol. In quiescent cells, present in very small amounts in the cytosol. Following induction of cell growth, first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum requires dephosphorylation at Tyr-10 and Tyr-30.

• Expression：
• Lung adenocarcinoma,Pancreas,Tongue,

NCX1 disturbs calcium homeostasis and promotes RANKL-induced osteoclast differentiation by regulating JNK/c-Fos/NFATc1 signaling pathway in multiple myeloma

Effect of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia in vitro MICROBIAL PATHOGENESIS Fengyi Zhou, Xin Li, Xiaochi Chang, Zhihao Geng, Wenjing Hao, Jing Deng, Hai Ming Wong, Shuai Wang WB Mouse BMMs

Ambient NO2 exposure induces migraine in rats: Evidence, mechanisms and interventions WB Rat /trigeminal nerve cells

Long-term effect of ropivacaine nanoparticles for sciatic nerve block on postoperative pain in rats. International Journal of Nanomedicine Int J Nanomed. 2016; 11: 2081–2090 IHC Rat 1:300 spinal

AP-1 confers resistance to anti-cancer therapy by activating XIAP. Oncotarget Oncotarget. 2018 Mar 6; 9(18): 14124–14137 WB Human MCF7 cell

The Effects of Maternal Atrazine Exposure and Swimming Training on Spatial Learning Memory and Hippocampal Morphology in Offspring Male Rats via PSD95/NR2B Signaling Pathway. CELLULAR AND MOLECULAR NEUROBIOLOGY Cell Mol Neurobiol. 2019 Oct;39(7):1003-1015 WB Rat 1:1000 hippocampus

Zhang, Huijuan, et al. "Musashi2 modulates K562 leukemic cell proliferation and apoptosis involving the MAPK pathway." Experimental cell research 320.1 (2014): 119-127.

JOURNAL OF HAZARDOUS MATERIALS Fang Xiao WB Rat 1:1000 trigeminal ganglion primary trigeminal ganglion neurons (PTGNs)

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