CARD 14 Polyclonal Antibody

    • Catalog No.:YT0636
    • Applications:IHC-p,IF,ELISA
    • Reactivity:Human,Mouse
      • Gene Name:
      • CARD14
      • Protein Name:
      • Caspase recruitment domain-containing protein 14
      • Human Swiss Prot No:
      • Q9BXL6
      • Mouse Swiss Prot No:
      • Q99KF0
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human CAR14. AA range:291-340
      • Specificity:
      • CARD 14 Polyclonal Antibody detects endogenous levels of CARD 14 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • CARD14; CARMA2; Caspase recruitment domain-containing protein 14; CARD-containing MAGUK protein 2; Carma 2
      • MolecularWeight(Da):
      • 113300
      • Observed Band(KD):
      • 110
      • Background:
      • caspase recruitment domain family member 14(CARD14) Homo sapiens This gene encodes a caspase recruitment domain-containing protein that is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. Members of this protein family are scaffold proteins that are involved in a diverse array of cellular processes including cellular adhesion, signal transduction and cell polarity control. This protein has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012],
      • Function:
      • caution:Supposed to contain a SH3 domain which is not detected by PROSITE, Pfam or SMART.,function:Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.,similarity:Contains 1 CARD domain.,similarity:Contains 1 guanylate kinase-like domain.,similarity:Contains 1 PDZ (DHR) domain.,subunit:CARD14 and BCL10 bind to each other by CARD-CARD interaction.,tissue specificity:Expressed in placenta. Also detected in HeLa S3 cells, but not in the other cancer cell lines tested.,
      • Subcellular Location:
      • cytoplasm,plasma membrane,
      • Expression:
      • Cervix,Colon,
      • Products Images
      • Immunofluorescence analysis of MCF7 cells, using CAR14 Antibody. The picture on the right is blocked with the synthesized peptide.