DAPK1 (Phospho Ser289) Rabbit pAb

    • Catalog No.:YP1813
    • Applications:IHC-p,WB
    • Reactivity:Human, Mouse
      • Fields:
      • >>Autophagy - animal;>>Pathways in cancer;>>Bladder cancer
      • Gene Name:
      • DAPK1 DAPK
      • Protein Name:
      • Death-associated protein kinase 1 (DAP kinase 1) (EC 2.7.11.1)
      • Sequence:
      • P53355
      • Human Gene Id:
      • 1612
      • Human Swiss Prot No:
      • P53355
      • Mouse Swiss Prot No:
      • Q80YE7
      • Immunogen:
      • Synthesized peptide derived from human DAPK1 (Phospho Ser289)
      • Specificity:
      • This antibody detects endogenous levels of DAPK1 (Phospho Ser289) Rabbit pAb at Human, Mouse
      • Formulation:
      • Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
      • Source:
      • Rabbit,polyclonal
      • Dilution:
      • WB 1:500-2000 IHC 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • Death-associated protein kinase 1 (DAP kinase 1) (EC 2.7.11.1)
      • Molecular Weight(Da):
      • 157kD
      • Background:
      • death associated protein kinase 1(DAPK1) Homo sapiens Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Negatively regulated by autophosphorylation on Ser-308.,function:Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.,induction:Up-regulated following treatment with interferon-gamma.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 death domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 10 ANK repeats.,subcellular location:Colocalizes with the actin filament system.,
      • Subcellular Location:
      • [Isoform 1]: Cytoplasm. Cytoplasm, cytoskeleton. Colocalizes with MAP1B in the microtubules and cortical actin fibers.; [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton.
      • Expression:
      • Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.
      • Products Images
      • Western Blot analysis of 1 HeLa cell, 2 LPS 100ng/mL 30min treated ,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000