TGFβ RI (phospho Ser165) Polyclonal Antibody

    • Catalog No.:YP1191
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • TGF β Receptor I
      • Fields:
      • >>MAPK signaling pathway;>>Cytokine-cytokine receptor interaction;>>FoxO signaling pathway;>>Endocytosis;>>Cellular senescence;>>TGF-beta signaling pathway;>>Apelin signaling pathway;>>Osteoclast differentiation;>>Hippo signaling pathway;>>Adherens junction;>>Th17 cell differentiation;>>Relaxin signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Chagas disease;>>Hepatitis B;>>Human T-cell leukemia virus 1 infection;>>Pathways in cancer;>>Colorectal cancer;>>Pancreatic cancer;>>Chronic myeloid leukemia;>>Hepatocellular carcinoma;>>Gastric cancer;>>Diabetic cardiomyopathy
      • Gene Name:
      • TGFBR1
      • Protein Name:
      • TGF-beta receptor type-1
      • Human Gene Id:
      • 7046
      • Human Swiss Prot No:
      • P36897
      • Mouse Swiss Prot No:
      • Q64729
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human TGF beta Receptor I around the phosphorylation site of Ser165. AA range:131-180
      • Specificity:
      • Phospho-TGFβ RI (S165) Polyclonal Antibody detects endogenous levels of TGFβ RI protein only when phosphorylated at S165.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500-2000, IF 1:50-300, IHC 1:50-300
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • TGFBR1;ALK5;SKR4;TGF-beta receptor type-1;TGFR-1;Activin A receptor type II-like protein kinase of 53kD;Activin receptor-like kinase 5;ALK-5;ALK5;Serine/threonine-protein kinase receptor R4;SKR4;TGF-beta type I receptor;Transfor
      • Molecular Weight(Da):
      • 56kD
      • Background:
      • The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008],
      • Function:
      • catalytic activity:ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.,cofactor:Magnesium or manganese.,disease:Defects in TGFBR1 are the cause of aortic aneurysm familial thoracic type 5 (AAT5) [MIM:608967]. Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.,disease:Defects in TGFBR1 are the cause of Loeys-Dietz syndrome type 1A (LDS1A) [MIM:609192]; also known as Furlong syndrome or Loeys-Dietz aortic aneurysm syndrome (LDAS). LDS1 is an aortic aneurysm syndrome with widespread systemic involvement. The disorder is characterized by arterial tort
      • Subcellular Location:
      • Cell membrane ; Single-pass type I membrane protein . Cell junction, tight junction . Cell surface . Membrane raft .
      • Expression:
      • Found in all tissues examined, most abundant in placenta and least abundant in brain and heart. Expressed in a variety of cancer cell lines (PubMed:25893292).
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,TGFβ RI (phospho Ser165) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Western Blot analysis of MOUSE-LIVER cells using Phospho-TGFβ RI (S165) Polyclonal Antibody diluted at 1:1000
      • Immunofluorescence analysis of HepG2 cells, Antibody diluted at 1:50. The picture on the right is blocked with the synthesized peptide.