Mcl-1 (phospho Ser159) Polyclonal Antibody

    • Catalog No.:YP1190
    • Applications:WB,IF,ELISA
    • Reactivity:Human,Mouse
      • Gene Name:
      • MCL1
      • Protein Name:
      • Induced myeloid leukemia cell differentiation protein Mcl-1
      • Human Gene Id:
      • 4170
      • Human Swiss Prot No:
      • Q07820
      • Mouse Swiss Prot No:
      • P97287
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human MCL1 around the phosphorylation site of Ser159. AA range:125-174
      • Specificity:
      • Phospho-Mcl-1 (S159) Polyclonal Antibody detects endogenous levels of Mcl-1 protein only when phosphorylated at S159.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • MCL1; BCL2L3; Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT
      • MolecularWeight(Da):
      • 37337
      • Observed Band(KD):
      • 39
      • Background:
      • BCL2 family apoptosis regulator(MCL1) Homo sapiens This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010],
      • Function:
      • function:Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis while isoform 2 promotes it.,induction:Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML-1. Rapidly up-regulated by CSF2 in ML-1 cells. Up-regulated by heat-shock induced differentiation. Expression increases early during retinoic acid-induced differentiation.,PTM:Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.,PTM:Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.,PTM:Rapidly degraded in the abs
      • Subcellular Location:
      • nucleus,nucleoplasm,cytoplasm,mitochondrion,mitochondrial outer membrane,mitochondrial matrix,cytosol,membrane,integral component of membrane,myosin complex,Bcl-2 family protein complex,
      • Expression:
      • Ewing sarcoma,Mammary gland,Myeloid leukemia cell,Neuroblastoma,Placenta,Th
      • Products Images
      • Western blot analysis of K562 using p-Mcl-1 (S159) antibody.
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MCL1 (Phospho-Ser159) Antibody
      • Immunofluorescence analysis of A549 cells, using MCL1 (Phospho-Ser159) Antibody. The picture on the right is blocked with the phospho peptide.