MyoD (phospho Ser200) Polyclonal Antibody

    • Catalog No.:YP0312
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • MyoD
      • Fields:
      • >>Spinocerebellar ataxia
      • Gene Name:
      • MYOD1
      • Protein Name:
      • Myoblast determination protein 1
      • Human Gene Id:
      • 4654
      • Human Swiss Prot No:
      • P15172
      • Mouse Swiss Prot No:
      • P10085
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human MYOD around the phosphorylation site of Ser200. AA range:171-220
      • Specificity:
      • Phospho-MyoD (S200) Polyclonal Antibody detects endogenous levels of MyoD protein only when phosphorylated at S200.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • MYOD1;BHLHC1;MYF3;MYOD;Myoblast determination protein 1;Class C basic helix-loop-helix protein 1;bHLHc1;Myogenic factor 3;Myf-3
      • Observed Band(KD):
      • 34kD
      • Background:
      • This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008],
      • Function:
      • function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.,online information:MyoD entry,PTM:Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.,PTM:Ubiquitinated on the N-terminus; which is required for proteasomal degradation.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1.,
      • Subcellular Location:
      • Nucleus.
      • Expression:
      • Muscle,Skeletal muscle,
      • Products Images
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MYOD (Phospho-Ser200) Antibody
      • Western blot analysis of lysates from Jurkat cells treated with Ca+ 40nM 30', using MYOD (Phospho-Ser200) Antibody. The lane on the right is blocked with the phospho peptide.
      • Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).