• Target：
• HMGB1

• Fields：
• >>Base excision repair;>>Autophagy - animal;>>Necroptosis;>>Neutrophil extracellular trap formation

• Gene Name：
• HMGB1 HMG1

• Protein Name：
• High mobility group protein B1 (High mobility group protein 1) (HMG-1)

• Human Gene Id：
• 3146

• Human Swiss Prot No：
• P09429

• Mouse Gene Id：
• 100862258

• Mouse Swiss Prot No：
• P63158

• Rat Gene Id：
• 25459

• Rat Swiss Prot No：
• P63159

• Specificity：
• endogenous

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Monoclonal, rabbit, IgG, Kappa

• Dilution：
• IHC 1:2000-1:10000;WB 1:5000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;

• Purification：
• Protein A

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• High mobility group protein B1 (High mobility group protein 1) (HMG-1)

• Molecular Weight(Da)：
• 25kD

• Observed Band(KD)：
• 25kD

• Background：
• high mobility group box 1(HMGB1) Homo sapiens This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015],

• Function：
• function:Binds preferentially single-stranded DNA and unwinds double stranded DNA.,similarity:Belongs to the HMGB family.,similarity:Contains 2 HMG box DNA-binding domains.,

• Subcellular Location：
• Nucleus

• Expression：
• Ubiquitous. Expressed in platelets (PubMed:11154118).

Huaier Induces Immunogenic Cell Death Via CircCLASP1/PKR/eIF2α Signaling Pathway in Triple Negative Breast Cancer WB Human /MDA-MB-231 cells

HMGB1 contributes to allergen-induced airway remodeling in a murine model of chronic asthma by modulating airway inflammation and activating lung fibroblasts. Cellular & Molecular Immunology Cell Mol Immunol. 2014 Aug;12(4):409-423 WB,IHC Mouse 1∶300 left lung

HMGB1 could restrict 1,3-β-glucan induced mice lung inflammation by affecting Beclin1 and Bcl2 interaction and promoting the autophagy of epithelial cells. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2021 Oct;222:112460 IF Mouse Lung

Hui, Zhi, and Zhe Li. "Continuous veno-venous hemofiltration for septic shock." INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 10.10 (2017): 14983-14988.

HMGB1 could restrict 1, 3-β-glucan induced mice lung inflammation by affecting Beclin1 and Bcl2 interaction and promoting the autophagy of epithelial cells." Ecotoxicology and Environmental Safety 222 (2021): 112460.

The protective role of HMGB1 in affecting the balance between autophagy and pyroptosis to maintain neutrophils homeostasis during β-glucan-induced mice lung inflammation ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Fangwei Liu IF Mouse lung

Multifunctional Metal-coordinated Co-assembled Carrier-free Nanoplatform Based on Dual-drugs for Ferroptosis-mediated Cocktail Therapy of Hepatocellular Carcinoma Growth and Metastasis JOURNAL OF COLLOID AND INTERFACE SCIENCE Rui-Rui Zhao IF Human HepG2 cell

Glucose regulates the HMGB1 signaling pathway through SIRT1 in glioma CELLULAR SIGNALLING Yu Wang WB,IF Human 1:1000,1:300 U87MG cell,LN229 cell

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