• Target：
• Bcl-2

• Fields：
• >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>Platinum drug resistance;>>NF-kappa B signaling pathway;>>HIF-1 signaling pathway;>>Sphingolipid signaling pathway;>>p53 signaling pathway;>>Autophagy - animal;>>Protein processing in endoplasmic reticulum;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Apoptosis - multiple species;>>Necroptosis;>>Adrenergic signaling in cardiomyocytes;>>Hedgehog signaling pathway;>>Focal adhesion;>>NOD-like receptor signaling pathway;>>JAK-STAT signaling pathway;>>Neurotrophin signaling pathway;>>Cholinergic synapse;>>Estrogen signaling pathway;>>Parathyroid hormone synthesis, secretion and action;>>AGE-RAGE signaling pathway in diabetic complications;>>Amyotrophic lateral sclerosis;>>Pathways of neurodegeneration - multiple diseases;>>Shigellosis;>>Salmonella infection;>>Toxoplasmosis;>>Tuberculosis;>>Hepatitis B;>>Measles;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Pathw

• Gene Name：
• BCL2

• Protein Name：
• Apoptosis regulator Bcl-2

• Human Gene Id：
• 596

• Human Swiss Prot No：
• P10415

• Mouse Gene Id：
• 12043

• Mouse Swiss Prot No：
• P10417

• Rat Gene Id：
• 24224

• Rat Swiss Prot No：
• P49950

• Specificity：
• endogenous

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Monoclonal, rabbit, IgG, Kappa

• Dilution：
• IHC 1:500-1:2000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;

• Purification：
• Protein A

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• BCL2;Apoptosis regulator Bcl-2

• Molecular Weight(Da)：
• 26kD

• Observed Band(KD)：
• 26kD

• Background：
• BCL2, apoptosis regulator(BCL2) Homo sapiens This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016],

• Function：
• disease:A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.,domain:The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.,function:Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activati

• Subcellular Location：
• Cytoplasm

• Expression：
• Expressed in a variety of tissues.

Maresin1 ameliorates ventricular remodelling and arrhythmia in mice models of myocardial infarction via NRF2/HO-1 and TLR4/NF-kB signalling

Decreased thioredoxin reductase 3 expression promotes nickel-induced damage to cardiac tissue via activating oxidative stress-induced apoptosis and inflammation

Preliminary verification of the anti-hypoxia mechanism of Gentiana straminea maxim based on UPLC-triple TOF MS/MS and network pharmacology

Effect of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia in vitro MICROBIAL PATHOGENESIS Fengyi Zhou, Xin Li, Xiaochi Chang, Zhihao Geng, Wenjing Hao, Jing Deng, Hai Ming Wong, Shuai Wang WB Mouse BMMs

Ursolic acid inhibits the activation of smoothened-independent non-canonical hedgehog pathway in colorectal cancer by suppressing AKT signaling cascade WB Human /HCT-116 cells

Shaoyao-Gancao Decoction Promoted Microglia M2 Polarization via the IL-13-Mediated JAK2/STAT6 Pathway to Alleviate Cerebral Ischemia-Reperfusion Injury WB Rat 1:500 penumbra tissue/

Synthesis of macromolecular Astragalus polysaccharide-nano selenium complex and the inhibitory effects on HepG2 cells WB Human /HepG2 cell

Design, Synthesis, and Apoptosis-Promoting Effect Evaluation of Rhopaladins’ Analog 4-Arylidene-5-Oxopyrrolidine Derivatives WB Human 1:1000 /HepG2 cell, 7402 cell

Genistein promotes M1 macrophage apoptosis and reduces inflammatory response by disrupting miR-21/TIPE2 pathway WB Mouse 1:1000 aortas /RAW264.7 cell

Melatonin treatment improves human umbilical cord mesenchymal stem cell therapy in a mouse model of type II diabetes mellitus via the PI3K/AKT signaling pathway. Stem Cell Research & Therapy2022 Dec;13(1):1-15. Human,Mouse 1:1200 liver tissue hUC-MSC

Licoflavone A Suppresses Gastric Cancer Growth and Metastasis by Blocking the VEGFR-2 Signaling Pathway. Journal of Oncology2022;2022:5497991. Human 1 : 1000 MKN-45 cell

LncRNA TUG1 Promotes Apoptosis, Invasion, and Angiogenesis of Retinal Endothelial Cells in Retinopathy of Prematurity via MiR-145-5p. Frontiers in Medicine2022 Apr 4;9:803214. Mouse 1:200 retinal tissue

hUC-MSCs lyophilized powder loaded polysaccharide ulvan driven functional hydrogel for chronic diabetic wound healing Carbohyd Polym. 2022 Jul;288:119404. IF Mouse 1:100 wound tissue

Bisphenol A impairs macrophages through inhibiting autophagy via AMPK/mTOR signaling pathway and inducing apoptosis Ecotox Environ Safe. 2022 Apr;234:113395. WB Mouse

An Integrated Approach Based on Network Analysis Combined With Experimental Verification Reveals PI3K/Akt/Nrf2 Signaling Is an Important Way for the Anti-Myocardial Ischemia Activity of Yi-Qi-Tong-Luo Capsule. Front Pharmacol. 2022 Feb;13:794528-794528. WB,IF Rat 1:1000

The Long-Noncoding RNA TUG1 Regulates Oxygen-Induced Retinal Neovascularization in Mice via MiR-299 Invest Ophth Vis Sci. 2022 Jan;63(1):37-37. WB Mouse 1:1000 retina

Banxia-Houpu decoction diminishes iron toxicity damage in heart induced by chronic intermittent hypoxia Pharm Biol. 2022;60(1):609-620. WB Mouse myocardial tissue

Moxibustion alleviates decreased ovarian reserve in rats by restoring the PI3K/AKT signaling pathway J Integr Med. 2022 Jan;:. IHC Rat Ovarian

Autophagy and apoptosis mediated nano-copper-induced testicular damage. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2022 Jan;229:113039 WB Rat Testis

Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro. Cell Death & Disease Cell Death Dis. 2017 Aug;8(8):e2966-e2966 WB Mouse 1:1000 ovarian tissue

Astragaloside IV Attenuates the Myocardial Injury Caused by Adriamycin by Inhibiting Autophagy. Frontiers in Pharmacology Front Pharmacol. 2021 May;0:1289 WB Rat 1 : 1500 Myocardial tissue

5-Lipoxygenase inhibition reduces inflammation and neuronal apoptosis via AKT signaling after subarachnoid hemorrhage in rats. Aging-US Aging-Us. 2021 Apr 30; 13(8): 11752–11761 WB Rat 1 : 1000 Brain

The inhibitory effects of selenium nanoparticles modified by fructose-enriched polysaccharide from Codonopsis pilosula on HepG2 cells. INDUSTRIAL CROPS AND PRODUCTS Ind Crop Prod. 2022 Feb;176:114335 WB Human HepG2 cell

Biased random walk model for the prioritization of drug resistance associated proteins. Scientific Reports 2015 Jun 03 WB Human 1:500 SGC7901 cell

Methane-Rich Saline Alleviates CA/CPR Brain Injury by Inhibiting Oxidative Stress, Microglial Activation-Induced Inflammatory Responses, and ER Stress-Mediated Apoptosis. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2020;2020:8829328 WB Rat 1:2000 Hippocampal

Hydrogen and Oxygen Mixture to Improve Cardiac Dysfunction and Myocardial Pathological Changes Induced by Intermittent Hypoxia in Rats. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2019;2019:7415212 WB Rat cardiac tissues

Polysaccharides from Pyracantha fortuneana and its biological activity. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Int J Biol Macromol. 2020 May;150:1162 WB Human Skov3 cell

Kaempferol potentiates the sensitivity of pancreatic cancer cells to erlotinib via inhibition of the PI3K/AKT signaling pathway and epidermal growth factor receptor. INFLAMMOPHARMACOLOGY Inflammopharmacology. 2021 Oct;29(5):1587-1601 WB Human PANC-1，BxPC-3

Production of Pyracantha Polysaccharide-Iron(III) Complex and Its Biologic Activity. MOLECULES Molecules. 2021 Jan;26(7):1949 WB Human SKOV3 cell

Zinc oxide nanoparticles induce apoptosis and autophagy in human ovarian cancer cells. International Journal of Nanomedicine Int J Nanomed. 2017; 12: 6521–6535 WB Human SKOV3 cell

Huperzine A, reduces brain iron overload and alleviates cognitive deficit in mice exposed to chronic intermittent hypoxia. LIFE SCIENCES Life Sci. 2020 Jun;250:117573 WB Mouse hippocampus

N′-[(3-[benzyloxy]benzylidene]-3,4,5-trihydroxybenzohydrazide (1) protects mice against colitis induced by dextran sulfate sodium through inhibiting NFκB/IL-6/STAT3 pathway. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Biochem Bioph Res Co. 2016 Aug;477:290 WB Mouse 1:1000 colon

Hydrogen ameliorates chronic intermittent hypoxia-induced neurocognitive impairment via inhibiting oxidative stress. BRAIN RESEARCH BULLETIN Brain Res Bull. 2018 Oct;143:225 WB Rat 1:1000 left Hippocampal

Raloxifene, a promising estrogen replacement, limits TDP-25 cell death by enhancing autophagy and suppressing apoptosis. BRAIN RESEARCH BULLETIN 2018 May 25 WB Human 1:500 TDP-25 cell

The Protective Effect of Aromatase on NSC-34 Cells with Stably Expressed hSOD1-G93A. NEUROSCIENCE Neuroscience. 2019 Jul;411:37 WB Human 1:500 hSOD1-G93A cell

Dopamine-Modified Zero-Valent Iron Nanoparticles for Dual-Modality Photothermal and Photodynamic Breast Cancer Therapy. ChemMedChem Chemmedchem. 2020 Sep;15(17):1645-1651 WB Human MCF-7 cell

Effect of annexin A7 suppression on the apoptosis of gastric cancer cells. MOLECULAR AND CELLULAR BIOCHEMISTRY Mol Cell Biochem. 2017 May;429(1):33-43 WB Human,Mouse BGC823 cell-xenograft BGC823 cell

Protective effects of flavonoids from the leaves of Carya cathayensis Sarg. against H₂O₂‑induced oxidative damage and apoptosis in vitro. Experimental and Therapeutic Medicine Exp Ther Med. 2021 Dec;22(6):1-11 WB Rat 1:1000 Rat aortic endothelial cells (RAECs)

Emodin suppresses the migration and invasion of melanoma cells. BIOLOGICAL & PHARMACEUTICAL BULLETIN 2021 Mar 17 WB Mouse,Human 1 : 500 B16F10 Melanoma Cells， A375 Melanoma Cells

The modified Yi qi decoction protects cardiac ischemia-reperfusion induced injury in rats. BMC Complementary and Alternative Medicine 2017 Jun 21 WB Rat 1:1000 Myocardial tissues (AAR tissue)

Exosome-mediated microRNA transfer plays a role in radiation-induced bystander effect. RNA Biology 2016 Jan 12 WB Human MRC5 cell

Soybean isoflavones prevent atrazine-induced neurodegenerative damage by inducing autophagy. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2020 Mar;190:110065 WB Rat 1:1000 substantia nigra,striatum

Notch signaling plays a crucial role in cancer stem-like cells maintaining stemness and mediating chemotaxis in renal cell carcinoma. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH J Exp Clin Canc Res. 2017 Dec;36(1):1-13 WB Human 1:500 RCC CSCs

HnRNP-L mediates bladder cancer progression by inhibiting apoptotic signaling and enhancing MAPK signaling pathways. Oncotarget Oncotarget. 2017 Feb 21; 8(8): 13586–13599 WB Human UM-UC-3 cell,EJ cell

MiR-21 is involved in radiation-induced bystander effects. RNA Biology 2015 Jan 26 WB MRC-5 cell

Protective effects of crocetin pretreatment on myocardial injury in an ischemia/reperfusion rat model. EUROPEAN JOURNAL OF PHARMACOLOGY Eur J Pharmacol. 2014 Oct;741:290 IHC Rat 1:150 heart

Polycyclic aromatic hydrocarbons are associated with increased risk of chronic obstructive pulmonary disease during haze events in China. SCIENCE OF THE TOTAL ENVIRONMENT 2016 Sep 07 WB Human 1:1000 BEAS-2B cell

The antitumor effect of hinesol, extract from Atractylodes lancea (Thunb.) DC. by proliferation, inhibition, and apoptosis induction via MEK/ERK and NF‐κB pathway in non–small cell lung cancer cell lines A549 and NCI‐H1299. JOURNAL OF CELLULAR BIOCHEMISTRY 2019 Jul 24 WB Human A549 cell

Hydrogen protects against chronic intermittent hypoxia induced renal dysfunction by promoting autophagy and alleviating apoptosis. LIFE SCIENCES Life Sci. 2019 May;225:46 WB Rat kidney

Resveratrol prevents chronic intermittent hypoxia-induced cardiac hypertrophy by targeting the PI3K/AKT/mTOR pathway. LIFE SCIENCES Life Sci. 2019 Sep;233:116748 WB Rat 1:1000 Myocardial tissues

Vangl2 is essential for myocardial remodeling activated by Wnt/JNK signaling. EXPERIMENTAL CELL RESEARCH Exp Cell Res. 2018 Apr;365:33 WB Rat cardiomyocytes

Cancer Stem Cells are Regulated by STAT3 Signalling in Wilms Tumour. Journal of Cancer J Cancer. 2018; 9(8): 1486–1499 WB Human,Mouse 1:1000 G401-Xenografts G401 cell

Long noncoding RNA LINC00152 promotes cell proliferation through competitively binding endogenous miR‐125b with MCL‐1 by regulating mitochondrial apoptosis pathways in ovarian cancer. Cancer Medicine Cancer Med-Us. 2018 Sep;7(9):4530-4541 WB Human,Mouse xenograft SKOV3 cell, A2780 cell

Reversing platinum resistance in ovarian cancer multicellular spheroids by targeting Bcl-2. OncoTargets and Therapy Oncotargets Ther. 2019; 12: 897–906 WB Human SKOV-3 cell, OVCAR-3 cell

Flupirtine attenuates chronic restraint stress-induced cognitive deficits and hippocampal apoptosis in male mice. BEHAVIOURAL BRAIN RESEARCH Behav Brain Res. 2015 Jul;288:1 WB Mouse 1:1000 hippocampus

Targeting FoxM1 by thiostrepton inhibits growth and induces apoptosis of laryngeal squamous cell carcinoma. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 2014 Nov 13 WB Human Hep-2 cell

Phosphoglycerate mutase 1 knockdown inhibits prostate cancer cell growth, migration, and invasion. ASIAN JOURNAL OF ANDROLOGY Asian J Androl. 2018 Mar-Apr; 20(2): 178–183 WB Human 1:500 PC-3 cell,22Rv1 cell,DU145 cell, LNCap cell

Atrazine Causes Autophagy- and Apoptosis-Related Neurodegenerative Effects in Dopaminergic Neurons in the Rat Nigrostriatal Dopaminergic System. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2015 Jun 12 WB Rat ventral midbrain

Olfactory Ensheathing Cell-Conditioned Medium Reverts Aβ 25–35 -Induced Oxidative Damage in SH-SY5Y Cells by Modulating the Mitochondria-Mediated Apoptotic Pathway. CELLULAR AND MOLECULAR NEUROBIOLOGY Cell Mol Neurobiol. 2017 Aug;37(6):1043-1054 IF Human 1:150 SH-SY5Y cell

LC3-II may mediate ATR-induced mitophagy in dopaminergic neurons through SQSTM1/p62 pathway. ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2018 Oct 01 WB Rat 1:1000 substantia nigra (SNpc) PC-12 cell

Rce1 expression in renal cell carcinoma and its regulatory effect on 786-O cell apoptosis through endoplasmic reticulum stress. ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2017 Mar 01 WB Human 1:2000 renal cancer tissues 786-O cell

Protective effect of luteolin on cigarette smoke extract‑induced cellular toxicity and apoptosis in normal human bronchial epithelial cells via the Nrf2 pathway. ONCOLOGY REPORTS Oncol Rep. 2014 Apr;31(4):1855-1862 WB Human NHBE cell

Total Flavonoids from Carya cathayensis Sarg. Leaves Alleviate H9c2 Cells Hypoxia/Reoxygenation Injury via Effects on miR-21 Expression, PTEN/Akt, and the Bcl-2/Bax Pathway. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2018;2018:8617314 WB Rat 1:1000 H9c2 cell

Glutamine ameliorates intestinal ischemia-reperfusion Injury in rats by activating the Nrf2/Are signaling pathway. International Journal of Clinical and Experimental Pathology Int J Clin Exp Patho. 2015; 8(7): 7896–7904 IHC Rat 1:100 intestinal

VX-680 induces p53-mediated apoptosis in human cholangiocarcinoma cells. ANTI-CANCER DRUGS Anti-Cancer Drug. 2018 Nov;29(10):1004-1010 WB Human 1 : 1000 HCCC-9810 cell

Combined effects of aspirin and vitamin D3 on two OSCC cell lines: a preliminary study. BIOTECHNOLOGY LETTERS 2018 Jan 18 WB Human CAL-27 cell,SCC-15 cell

Down-regulation of FoxM1 by thiostrepton or small interfering RNA inhibits proliferation, transformation ability and angiogenesis, and induces apoptosis of nasopharyngeal carcinoma cells. International Journal of Clinical and Experimental Pathology Int J Clin Exp Patho. 2014; 7(9): 5450–5460 WB Human Nasopharyngeal carcinoma (NPC) cell

Synergistic Apoptotic Effect of D-Fraction From Grifola frondosa and Vitamin C on Hepatocellular Carcinoma SMMC-7721 Cells:. INTEGRATIVE CANCER THERAPIES 2016 May 05 WB Human SMMC-7721 cell

Exosome-derived microRNA-29c Induces Apoptosis of BIU-87 Cells by Down Regulating BCL-2 and MCL-1. Asian Pacific Journal of Cancer Prevention 2014 Apr 30 IHC Human Bladder Cancer tissue

Effect of metformin on apoptosis, cell cycle arrest migration and invasion of A498 cells. Molecular Medicine Reports 2014 Mar 31 WB Human A498 cell

Protective effects of tanshinone IIA sodium sulfonate on ischemia-reperfusion-induced myocardial injury in rats. Iranian Journal of Basic Medical Sciences Iran J Basic Med Sci. 2017 Mar; 20(3): 308–315 WB Rat 1:1000 Myocardial tissues

Apoptosis triggered by pyropheophorbide-α methyl ester-mediated photodynamic therapy in a giant cell tumor in bone. LASER PHYSICS Laser Phys. 2014 May;24(6):066002 WB Human SGC-0404 cell

miR-2954 Inhibits PI3K Signaling and Induces Autophagy and Apoptosis in Myocardium Selenium Deficiency. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 2018 Nov 21 WB Chicken 1:500 cardiomyocytes

Effects of Ovariectomy in an hSOD1-G93A Transgenic Mouse Model of Amyotrophic Lateral Sclerosis (ALS). MEDICAL SCIENCE MONITOR Med Sci Monitor. 2018; 24: 678–686 WB Mouse 1: 500 spinal cord

Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang Improves Cardiac Function after Myocardial Infarction in Rats. Evidence-based Complementary and Alternative Medicine 2020 Oct 09 WB Rat 1 : 1000 Myocardial tissue

Mitochondrial separation protein inhibitor inhibits cell apoptosis in rat lungs during intermittent hypoxia. Experimental and Therapeutic Medicine 2019 Mar 01 WB Human,Rat WRTL1 cell

Fang, Zhiqing, et al. "Nitidine chloride induces apoptosis and inhibits tumor cell proliferation via suppressing ERK signaling pathway in renal cancer." Food and chemical toxicology 66 (2014): 210-216.

Fang, Zhiqing, et al. "Simvastatin inhibits renal cancer cell growth and metastasis via AKT/mTOR, ERK and JAK2/STAT3 pathway." PloS one 8.5 (2013): e62823.

N′-[(3-[benzyloxy] benzylidene]-3, 4, 5-trihydroxybenzohydrazide (1) protects mice against colitis induced by dextran sulfate sodium through inhibiting NFκB/IL-6/STAT3 pathway." Biochemical and biophysical research communications 477.2 (2016): 290-296.

Suppression of Cellular Proliferation and Invasion by HMGB1 Knockdown in Bladder Urothelial Carcinoma Cells. ONCOLOGY RESEARCH Oncol Res. 2015; 22(5-6): 235–245 WB Human BIU-87 cell, T24 cell

Astragaloside IV Attenuates the Myocardial Injury Caused by Adriamycin by Inhibiting Autophagy. Frontiers in Pharmacology Front Pharmacol. 2021 May;0:1289 WB Rat 1 : 1500 Myocardial tissue

Flavonoid-Rich Extract of Oldenlandia diffusa (Willd.) Roxb. Inhibits Gastric Cancer by Activation of Caspase-Dependent Mitochondrial Apoptosis Chinese Journal of Integrative Medicine Qiu-lan Wang WB Human

Establishment of fingerprint and mechanism of anti-myocardial ischemic effect of Syringa pinnatifolia BIOMEDICAL CHROMATOGRAPHY Peifeng Xue WB Mouse

Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3 Oxidative Medicine and Cellular Longevity Congxin Huang WB Mouse heart tissue H9c2 cells

Shikonin potentiates therapeutic efficacy of oxaliplatin through reactive oxygen species-mediated intrinsic apoptosis and endoplasmic reticulum stress in oxaliplatin-resistant colorectal cancer cells DRUG DEVELOPMENT RESEARCH Yajie Zhang WB Human,Mouse HCT116R-xenograft OXA-resistant CRC cells (HCT116R)

Preliminary Structural Characterization of Selenium Nanoparticle Composites Modified by Astragalus Polysaccharide and the Cytotoxicity Mechanism on Liver Cancer Cells MOLECULES Xiaoyu Jia WB Human HepG2 cell

Melatonin alleviates doxorubicin-induced cardiotoxicity via inhibiting oxidative stress, pyroptosis and apoptosis by activating Sirt1/Nrf2 pathway BIOMEDICINE & PHARMACOTHERAPY Congxin Huang WB Rat,Mouse heart H9C2 cell

TAK-715 alleviated IL-1β-induced apoptosis and ECM degradation in nucleus pulposus cells and attenuated intervertebral disc degeneration ex vivo and in vivo ARTHRITIS RESEARCH & THERAPY Lin Huang IHC,WB Rat disc samples,nucleus pulposus cells (NPCs)samples

MicroRNA-155 suppressed cholesterol-induced matrix degradation, pyroptosis and apoptosis by targeting RORα in nucleus pulposus cells CELLULAR SIGNALLING Wei Ye IHC Rat nucleus pulposus (NP) tissue

Comparison of two kinds of Agrocybe cylindracea polysaccharides: structural characteristic and antitumor activity. An-jun Liu WB Human MGC-803 cell

Preventive effect of LCZ696 on hypoxic pulmonary hypertension in rats via regulating the PI3K/AKT signaling pathway. Ya-Feng Wang WB Rat 1:1000 Lung tissue

Dangshen Huangjiu exerts prevention of gastric mucosal injury and inhibits Akt/NF-κB signaling. Fangdi Hu WB Rat gastric mucosal tissues

Celastrol attenuates diabetic nephropathy by upregulating SIRT1-mediated inhibition of EZH2 related wnt/β-catenin signaling. Ruchun Yang WB Mouse podocytes, MPC5 cell

Indisulam exerts anticancer effects via modulation of transcription, translation and alternative splicing on human cervical cancer cells. Cuixia Di WB Human 1:1000 HeLa cell

Itaconic acid induces angiogenesis and suppresses apoptosis via Nrf2/autophagy to prolong the survival of multi-territory perforator flaps. Liming Qing WB Rat 1:1000 flap tissue

Dietary antioxidant quercetin overcomes the acquired resistance of Sorafenib in Sorafenib-resistant hepatocellular carcinoma cells through epidermal growth factor receptor signaling inactivation. Zhou Fuqiong WB Mouse，Human 1:1000 Huh7 cell-xenograft Huh7 cell

Banxia-Houpu decoction inhibits iron overload and chronic intermittent hypoxia-induced neuroinflammation in mice. JOURNAL OF ETHNOPHARMACOLOGY Yashuo Zhao WB Mouse hippocampal

Role of Txnrd3 in NiCl2-induced kidney cell apoptosis in mice: Potential therapeutic effect of melatonin. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ziwei Zhang WB Mouse 1:2000 kidney

Protective effect of cornuside on OGD/R injury in SH-SY5Y cells and its underlying mechanism. BRAIN RESEARCH Jianwei Gong WB Human SH-SY5Y cell

L-Theanine delays D-galactose-induced senescence by regulating the cell cycle and inhibiting apoptosis in rat intestinal cells JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE Wei Xu WB Rat 1:2000 IEC-6 cell

The role of vitamin D through SphK1/S1P in the regulation of MS progression JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY Zhen Wang WB Rat PC12 cell

maresin2 fine-tunes ULK1 O-GlcNAcylation to improve post myocardial infarction remodeling EUROPEAN JOURNAL OF PHARMACOLOGY Jingjing Zhang WB Mouse,Rat 1:1000 heart tissue neonatal rat cardiomyocytes (NRCMs)

Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Mengting Xu WB Mouse,Human 1:1000 ovarian tissue KGN cell

Roflupram alleviates autophagy defects and reduces mutant hSOD1-induced motor neuron damage in cell and mouse models of amyotrophic lateral sclerosis NEUROPHARMACOLOGY Di Huo WB Mouse 1:1000 spinal cord tissue NSC34 cell

Synthesis of peiminine derivatives and its effect on A549 lung cancer-bearing mice Chemical Biology & Drug Design Xin Jin WB Mouse A549 cell-Xenograft

L-theanine protects against D-galactose-induced aging in L6 skeletal muscle cells Journal of Functional Foods Min Long WB Rat 1:2000 L6 cell

ADPN Regulates Oxidative Stress-Induced Follicular Atresia in Geese by Modulating Granulosa Cell Apoptosis and Autophagy INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Yan Zheng WB Goose 1:1000 follicular tissue

Lung decellularized matrix-derived 3D spheroids: Exploring silicosis through the impact of the Nrf2/Bax pathway on myofibroblast dynamics Heliyon Wenming Xue IF,WB Mouse 1:1000 NIH/3T3 cell

Delivery of 5-fluorouracil for cancer therapy using aptamer-based nonlinear hybridization chain reaction INTERNATIONAL JOURNAL OF PHARMACEUTICS Haofei Ji WB Human HepG-2 cell

Neuroprotective effects of total phenolics from Hemerocallis citrina Baroni leaves through the PI3K/AKT pathway Frontiers in Pharmacology Jia Yanjun WB Rat PC12 cell

Gastrodin against oxidative stress-inflammation crosstalk via inhibiting mtDNA/TLR9 and JAK2/STAT3 signaling to ameliorate ischemic stroke injury INTERNATIONAL IMMUNOPHARMACOLOGY Menglian Zhang WB Rat 1:2000 brain microvascular endothelial cell (BMEC)

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs