His Tag (PT0481R) PT® Rabbit mAb
- Catalog No.:YM8314
- Applications:WB;IF;IP;ELISA
- Target:
- His-Tag
- Gene Name:
- His-Tag
- Protein Name:
- His Tag
- Specificity:
- endogenous
- Formulation:
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- Source:
- Monoclonal, rabbit, IgG, Kappa
- Dilution:
- WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;
- Purification:
- Protein A
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Molecular Weight(Da):
- 50kD
- Observed Band(KD):
- 55kD
- Background:
- A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Atractylenolide-I covalently binds to CYP11B2, selectively inhibits aldosterone synthesis, and improves hyperaldosteronism. Acta Pharmaceutica Sinica B Acta Pharm Sin B. 2021 Sep;: WB Human NCI-H295R cell
Development and characterization of in vitro self-assembled recombinant human follicle stimulating hormone originated from goat mammary epithelial cells. MOLECULAR AND CELLULAR ENDOCRINOLOGY Mol Cell Endocrinol. 2021 Apr;526:111211 Pull down Hamster 1:16000 Chinese hamster ovary (CHO) cell
TEAD4 functions as a prognostic biomarker and triggers EMT via PI3K/AKT pathway in bladder cancer WB Human 1:10000 /V-HUC-1 cell, 5637 cell,, BIU-87 cell,, T24 cell,, TCCSUP cell
Cloning and functional analysis of the VcCXIP4 and VcYSL6 genes as Cd-regulating genes in blueberry. GENE Gene. 2019 Feb;686:104 WB Blueberry 1:5000 leaves
Betulin efficiently suppresses the process of an experimental Listeria monocytogenes infection as an antagonist against listeriolysin O. FITOTERAPIA Fitoterapia. 2019 Nov;139:104409 WB Listeria monocytogenes
Uncovering the efficacy and mechanisms of Genkwa flos and bioactive ingredient genkwanin against L. monocytogenes infection JOURNAL OF ETHNOPHARMACOLOGY Lei Xu WB Listeria monocytogenes
Structures of the CcmABCD heme release complex at multiple states
The BnTFL1–BnGF14nu–BnFD module regulates flower development and plant architecture in Brassica napus. Crop Journal Mingxun Chen WB,Pull Down Escherichia coli 1:2000
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- Antibody-FAQs
- Products Images
- Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-His Tag (PT0481R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Flag-His-MBP Lane 2: Myc-His-MBP Lane 3: HA-His-MBP Predicted band size: 50kDa Observed band size: 55kDa