• Target：
• TNFA

• Fields：
• >>Antifolate resistance;>>MAPK signaling pathway;>>Cytokine-cytokine receptor interaction;>>Viral protein interaction with cytokine and cytokine receptor;>>NF-kappa B signaling pathway;>>Sphingolipid signaling pathway;>>mTOR signaling pathway;>>Apoptosis;>>Necroptosis;>>TGF-beta signaling pathway;>>Osteoclast differentiation;>>Antigen processing and presentation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>Hematopoietic cell lineage;>>Natural killer cell mediated cytotoxicity;>>IL-17 signaling pathway;>>T cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>TNF signaling pathway;>>Adipocytokine signaling pathway;>>Type II diabetes mellitus;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>AGE-RAGE signaling pathway in diabetic complications;>>Alcoholic liver disease;>>Type I diabetes mellitus;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>P

• Gene Name：
• TNF TNFA TNFSF2

• Protein Name：
• Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF); Intracellular

• Human Gene Id：
• 7124

• Human Swiss Prot No：
• P01375

• Mouse Gene Id：
• 21926

• Mouse Swiss Prot No：
• P06804

• Rat Gene Id：
• 24835

• Rat Swiss Prot No：
• P16599

• Specificity：
• endogenous

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Monoclonal, rabbit, IgG, Kappa

• Dilution：
• IHC 1:2000-1:10000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;

• Purification：
• Protein A

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• TNF;TNFA;TNFSF2;Tumor necrosis factor;Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a

• Molecular Weight(Da)：
• 26kD

• Observed Band(KD)：
• 18kD

• Background：
• This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008],

• Function：
• disease:Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition.,disease:Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.,disease:Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated w

• Subcellular Location：
• Membrane

• Expression：
• Blood,Peripheral blood leukocyte,Placenta,Prostatic carcinoma,

Maresin1 ameliorates ventricular remodelling and arrhythmia in mice models of myocardial infarction via NRF2/HO-1 and TLR4/NF-kB signalling

New injectable chitosan-hyaluronic acid based hydrogels for hemostasis and wound healing IF Mouse /granulation tissues

Melatonin treatment improves human umbilical cord mesenchymal stem cell therapy in a mouse model of type II diabetes mellitus via the PI3K/AKT signaling pathway. Stem Cell Research & Therapy2022 Dec;13(1):1-15. Mouse 1:100 liver, pancreas, white adipose tissue

LncRNA TUG1 Promotes Apoptosis, Invasion, and Angiogenesis of Retinal Endothelial Cells in Retinopathy of Prematurity via MiR-145-5p. Frontiers in Medicine2022 Apr 4;9:803214. Mouse 1:200 retinal tissue

Mechanical loading mitigates osteoarthritis symptoms by regulating the inflammatory microenvironment in a mouse model 2022 Feb 27. WB,IHC Mouse 1:200 articular cartilage

The Long-Noncoding RNA TUG1 Regulates Oxygen-Induced Retinal Neovascularization in Mice via MiR-299 Invest Ophth Vis Sci. 2022 Jan;63(1):37-37. WB,IF,IHC Mouse 1:1000,1:200,1:200 retina

Single cell derived spheres of umbilical cord mesenchymal stem cells enhance cell stemness properties, survival ability and therapeutic potential on liver failure. BIOMATERIALS 2019 Oct 21 IHC,IF Mouse liver ,xenograft

Human Novel MicroRNA Seq-915_x4024 in Keratinocytes Contributes to Skin Regeneration by Suppressing Scar Formation. Molecular Therapy-Nucleic Acids Mol Ther-Nucl Acids. 2019 Mar;14:410 WB Human HaCaT cell

Adipokine expression and endothelial function in subclinical hypothyroidism rats. Endocrine Connections Endocr Connect. 2018 Feb;7(2):295-304 WB Rat 1:1000 visceral adipose tissue (VAT)

The Change of Left Ventricular Function in Rats with Subclinical Hypothyroid and the Effects of Thyroxine Replacement. International Journal of Endocrinology Int J Endocrinol. 2018;2018:8682765 WB,IHC Rat 1 : 200,1 : 200 heart

Flavonoid-Rich Ethanol Extract from the Leaves of Diospyros kaki Attenuates D-Galactose-Induced Oxidative Stress and Neuroinflammation-Mediated Brain Aging in Mice. Oxidative Medicine and Cellular Longevity 2018 Dec 23 WB Mouse 1 : 1000 cortex,hippocampus

Flavonoid-rich ethanol extract from the leaves of Diospyros kaki attenuates cognitive deficits, amyloid-beta production, oxidative stress, and neuroinflammation in APP/PS1 transgenic mice. BRAIN RESEARCH Brain Res. 2018 Jan;1678:85 WB Mouse 1:1000 Brain

DJ-1 Regulates Microglial Polarization Through P62-Mediated TRAF6/IRF5 Signaling in Cerebral Ischemia-Reperfusion. Frontiers in Cell and Developmental Biology Front Cell Dev Biol. 2020; 8: 593890 WB Rat 1:200 Ischemic penumbral tissues HAPI cell

Maternally expressed gene 3 regulates retinal neovascularization in retinopathy of prematurity. Neural Regeneration Research Neural Regen Res. 2022 Jun; 17(6): 1364–1368 WB Mouse 1:1000 Retina

Simvastatin Enhances Muscle Regeneration Through Autophagic Defect-Mediated Inflammation and mTOR Activation in G93ASOD1 Mice. MOLECULAR NEUROBIOLOGY Mol Neurobiol. 2021 Apr;58(4):1593-1606 IF Mouse 1:100,1:1000 Muscle

Attenuation of Inflammation by DJ-1 May Be a Drug Target for Cerebral Ischemia–Reperfusion Injury. NEUROCHEMICAL RESEARCH Neurochem Res. 2021 Jun;46(6):1470-1479 WB Rat 1:300 Cerebral cortex tissue

SANT, a novel Chinese herbal monomer combination, decreasing tumor growth and angiogenesis via modulating autophagy in heparanase overexpressed triple-negative breast cancer. JOURNAL OF ETHNOPHARMACOLOGY J Ethnopharmacol. 2021 Feb;266:113430 WB Human MDA-MB-231 hpa (231-Hpa)cell-Xenograft,MDA-MB-231 mock (231-Mock) cell-Xenograft

Lipopolysaccharide Promotes Inflammatory Response via Enhancing IFIT1 Expression in Human Umbilical Vein Endothelial Cells. DNA AND CELL BIOLOGY Dna Cell Biol. 2020 Jul;39(7):1274-1281 WB Human HUVECs

The potentially protective role of ATP-binding cassette transporters in preeclampsia via Nrf2. Pregnancy Hypertension-An International Journal of Womens Cardiovascular Health WB Human placental

Vagus Nerve Stimulation Transiently Mitigates Chemotherapy-Induced Peripheral Neuropathy in Rats. Journal of Pain Research J Pain Res. 2020; 13: 3457–3465 WB Rat 1:1000 Dorsal root ganglion

Dissecting the roles of Ephrin-A3 in malignant peripheral nerve sheath tumor by TALENs. ONCOLOGY REPORTS Oncol Rep. 2015 Jul;34(1):391-398 WB Human ST88-14 cell, sNF96.2 cell

Small Peptides Compound Isolated from Agkistrodon with Antiarthritic Effect in Collagen-Induced Arthritis Rats. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2018;2018:8265150 IHC Rat hind limb

Crassifoside H ameliorates depressant behavior in chronic unpredictable mild stress rats by improving HPA axis dysfunction and inhibiting inflammation in hippocampus. TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH Trop J Pharm Res. 2020 Nov;19(8):1693-1699 WB 1 : 1000 Hippocampus sample

Jiang, Kang-feng, et al. "Polydatin ameliorates Staphylococcus aureus-induced mastitis in mice via inhibiting TLR2-mediated activation of the p38 MAPK/NF-κB pathway." Acta Pharmacologica Sinica 38.2 (2017): 211.

Hui, Zhi, and Zhe Li. "Continuous veno-venous hemofiltration for septic shock." INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 10.10 (2017): 14983-14988.

Monti, Daria M. "Role of Antioxidants in the Protection from Aging-Related Diseases."

Patchouli oil isolated from the leaves of Pogostemon cablin ameliorates ethanol-induced acute liver injury in rats via inhibition of oxidative stress and lipid accumulation. RSC Advances Rsc Adv. 2018 Jul;8(43):24399-24410 ELISA Rat Liver tissues

Immunomodulatory effect and safety of TNF-α RNAi mediated by oral yeast microcapsules in rheumatoid arthritis therapy Materials Today Bio Long Zhang IF Rat

Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3 Oxidative Medicine and Cellular Longevity Congxin Huang WB Mouse heart tissue H9c2 cells

Spleen Toxicity of Organophosphorus Flame Retardant TDCPP in Mice and the Related Mechanisms Toxics Kaihua Liu WB Mouse spleen tissues

A novel microbial and hepatic biotransformation-integrated network pharmacology strategy explores the therapeutic mechanisms of bioactive herbal products in neurological diseases: the effects of Astragaloside IV on intracerebral hemorrhage as an example Chinese Medicine Yang Wang IF Mouse brain

A biodegradable, flexible photonic patch for in vivo phototherapy. Yang Zhu IHC Rat 1:200 heart

Suppression of TGFβR-Smad3 pathway alleviates the syrinx induced by syringomyelia. Fengzeng Jian IF Rat spinal cord

CXCR2 antagonism attenuates neuroinflammation after subarachnoid hemorrhage. Xiaolin Luo WB Mouse 1:500 brain tissue

The effects of astragaloside IV on gut microbiota and serum metabolism in a mice model of intracerebral hemorrhage. PHYTOMEDICINE Yang Wang WB Mouse 1:1000 brain

Production of recombinant human long-acting IL-18 binding protein: inhibitory effect on ulcerative colitis in mice. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY Xiao Yechen WB Mouse colonic tissues

AAV2-antiVEGFscFv gene therapy for retinal neovascularization. Molecular Therapy-Methods & Clinical Development Guangzuo Luo IHC Mouse eyes

A self-assembled hydrogel dressing as multi-target therapeutics to promote wound healing CHEMICAL ENGINEERING JOURNAL Yao Wu IHC Rat skin wound tissue

Week-long norm glycaemia in diabetic mice and minipigs via a subcutaneous dose of a glucose-responsive insulin complex Nature Biomedical Engineering Zhang Juan IHC Mouse 1:200 skin tissue

Knockdown of Galectin-9 alleviates rheumatoid arthritis through suppressing TNF-α-induced activation of fibroblast-like synoviocytes BIOCHEMICAL PHARMACOLOGY Qian Jia IHC,IF Human 1:200,1:200 synovial tissue fibroblasts

Mechanism of NO2-induced migraine in rats: The exploration of the role of miR-653-3p/IGF1 axis JOURNAL OF HAZARDOUS MATERIALS Shuzi Ye WB Rat 1:1000 trigeminal ganglion primary trigeminal ganglion neurons (PTGNs)

MicroRNA-181b attenuates lipopolysaccharide-induced inflammatory responses in pulpitis via the PLAU/AKT/NF-κB axis INTERNATIONAL IMMUNOPHARMACOLOGY Tiantian Meng WB Human 1:1000 human dental pulp cells (hDPCs)

Rosmarinic acid mitigates acrylamide induced neurotoxicity via suppressing endoplasmic reticulum stress and inflammation in mouse hippocampus PHYTOMEDICINE Tian-Bao Wang IHC,WB Mouse hippocampal

Protective Effect of Mesenchymal Stem Cell Active Factor Combined with Alhagi maurorum Extract on Ulcerative Colitis and the Underlying Mechanism INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Xuanhong Cao WB Mouse 1:1200 colon tissue

ω3-PUFA alleviates neuroinflammation by upregulating miR-107 targeting PIEZO1/NFκB p65 INTERNATIONAL IMMUNOPHARMACOLOGY Hailin Liu WB Mouse 1:1000 hippocampus tissue

Multifunctional Drug- and AuNRs-Loaded ROS-Responsive Selenium-Containing Polyurethane Nanofibers for Smart Wound Healing ACS Biomaterials Science & Engineering Wajiha Ahmed IHC Rat skin tissue

MiR-21-5p modulates LPS-induced acute injury in alveolar epithelial cells by targeting SLC16A10 Scientific Reports Zeng Huanan WB Human 1:1000 A549 cell

Neuroprotective effects of total phenolics from Hemerocallis citrina Baroni leaves through the PI3K/AKT pathway Frontiers in Pharmacology Jia Yanjun WB Rat PC12 cell

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs