• Target：
• AKT1/2/3

• Fields：
• >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>Platinum drug resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Apoptosis;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Adrenergic signaling in cardiomyocytes;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Osteoclast differentiation;>>Focal adhesion;>>Signaling pathways regulating pluripotency of stem cells;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>JAK-STAT signaling pathway;>>T cell recept

• Gene Name：
• AKT1/AKT2/AKT3

• Protein Name：
• RAC-alpha serine/threonine-protein kinase/RAC-beta serine/threonine-protein kinase/RAC-gamma serine/threonine-protein kinase

• Human Gene Id：
• 207/208/10000

• Human Swiss Prot No：
• P31749;P31751;Q9Y243

• Mouse Gene Id：
• 11651/11652/23797

• Rat Gene Id：
• 24185/25233/29414

• Rat Swiss Prot No：
• P47196/P47197/Q63484

• Specificity：
• endogenous

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Monoclonal, rabbit, IgG, Kappa

• Dilution：
• IHC 1:200-1:500;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;

• Purification：
• Protein A

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• AKT1;PKB;RAC;RAC-alpha serine/threonine-protein kinase;Protein kinase B;PKB;Protein kinase B alpha;PKB alpha;Proto-oncogene c-Akt;RAC-PK-alpha;AKT2;RAC-beta serine/threonine-protein kinase;Protein kinase Akt-2;Protein kinase B

• Molecular Weight(Da)：
• 55kD

• Observed Band(KD)：
• 60kD

• Background：
• The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in AKT1 are associated with breast cancer (BC) [MIM:114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime.,disease:Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].,disease:Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).,domain:Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.,domain:The AGC-kinase C-terminal mediates interaction with THEM4.,enzyme regulation:Three specific sites, one in the kinase domain (Thr-308) and the two other ones in the C-terminal regulatory region (Ser-473 and Tyr-474), need to be phosphorylated for its full activation.,function:Gene

• Subcellular Location：
• Cytoplasm

• Expression：
• Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.

CFNC, a neocryptolepine derivative, inhibited the growth of gastric cancer AGS cells by inhibiting PI3K/AKT signaling pathway

Pregabalin Mediates Retinal Ganglion Cell Survival From Retinal Ischemia/Reperfusion Injury Via the Akt/GSK3β/β-Catenin Signaling Pathway

MiR-140-3p directly targets Tyro3 to regulate OGD/R-induced neuronal injury through the PI3K/Akt pathway

Combination of chloroquine diphosphate and salidroside induces human liver cell apoptosis via regulation of mitochondrial dysfunction and autophagy Molecular Medicine Reports Bing Jiang, Longfei Feng, Tao Yang, Wenjing Guo, Yangyang Li, Tao Wang, Chengguang Liu, Haixiang Su WB Human 97H cell

MicroRNA-10 Family Promotes the Epithelial-to-Mesenchymal Transition in Renal Fibrosis by the PTEN/Akt Pathway CURRENT ISSUES IN MOLECULAR BIOLOGY Chaokun Wang, Yichen Shuai, Chuan Zhao, Fengrui Yang, Weilian Su, Zhifen Ning, Guoxia Li WB Human HK-2 cell

Ursolic acid inhibits the activation of smoothened-independent non-canonical hedgehog pathway in colorectal cancer by suppressing AKT signaling cascade WB Human /HCT-116 cells

Synthesis and anticancer evaluations of novel 1H-imidazole [4,5-f][1,10] phenanthroline derivative for the treatment of colorectal cancer WB Human /HCT116, SW480 cells

Proteomic Study on the Reproductive Toxicity of Tripterygium Glycosides in Rats WB Rat testis tissue /

Genistein promotes M1 macrophage apoptosis and reduces inflammatory response by disrupting miR-21/TIPE2 pathway WB Mouse 1:1000 aortas/RAW264.7 cell

Irisin Suppresses Nicotine-Mediated Atherosclerosis by Attenuating Endothelial Cell Migration, Proliferation, Cell Cycle Arrest, and Cell Senescence. Frontiers in Cardiovascular Medicine2022; 9: 851603. Human 1:4000 HUVECs

Design, synthesis, and cytotoxic activities of isaindigotone derivatives as potential anti-gastric cancer agents. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY2022;37(1):1212-1226. Human AGS cell

Licoflavone A Suppresses Gastric Cancer Growth and Metastasis by Blocking the VEGFR-2 Signaling Pathway. Journal of Oncology2022;2022:5497991. Human 1 : 1000 MKN-45 cell

Network Pharmacology-Based Strategy to Identify the Pharmacological Mechanisms of Pulsatilla Decoction against Crohn's Disease.. Frontiers in Pharmacology2022 Apr;13:844685-844685. Human,Mouse 1:1000 colon tissues

An Integrated Approach Based on Network Analysis Combined With Experimental Verification Reveals PI3K/Akt/Nrf2 Signaling Is an Important Way for the Anti-Myocardial Ischemia Activity of Yi-Qi-Tong-Luo Capsule. Front Pharmacol. 2022 Feb;13:794528-794528. WB,IF Rat 1:1000

Mammalian elongation factor 4 regulates mitochondrial translation essential for spermatogenesis. NATURE STRUCTURAL & MOLECULAR BIOLOGY 2016 Apr 11 WB Human gastric cancer tissues

Insulin Attenuates Beta-Amyloid-Associated Insulin/Akt/EAAT Signaling Perturbations in Human Astrocytes. CELLULAR AND MOLECULAR NEUROBIOLOGY Cell Mol Neurobiol. 2016 Aug;36(6):851-864 WB Human 1:1000 Astrocytes

Cystamine attenuated behavioral deficiency via increasing the expression of BDNF and activating PI3K/Akt signaling in 2,5-hexanedione intoxicated rats. Toxicology Research 2017 Mar 01 WB Rat spinal cord

Deletion of FGF9 in GABAergic neurons causes epilepsy. Cell Death & Disease Cell Death Dis. 2021 Feb;12(2):1-13 WB Mouse Brain

Autophagy and apoptosis mediated nano-copper-induced testicular damage. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2022 Jan;229:113039 WB Rat Testis

circCDYL2, Overexpressed in Highly Migratory Colorectal Cancer Cells, Promotes Migration by Binding to Ezrin. Frontiers in Oncology Front Oncol. 2021; 11: 716073 WB Human 1:1000,1:200 CRC tissue SW620, RKOSW480,HCT116

Over-Expression of Centromere Protein U Participates in the Malignant Neoplastic Progression of Breast Cancer. Frontiers in Oncology Front Oncol. 2021 Mar;0:350 WB Human 1:1000 Breast cancer tissues ，the paired adjacent histologically normal tissue T47D cell, MDA-MB-231 cell, SK-BR-3 cell, MCF-7 cell

Isorhamnetin Promotes MKN-45 Gastric Cancer Cell Apoptosis by Inhibiting PI3K-Mediated Adaptive Autophagy in a Hypoxic Environment. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY J Agr Food Chem. 2021;XXXX(XXX):XXX-XXX WB Human MKN-45 cells

5‐FU inhibits migration and invasion of CRC cells through PI3K/AKT pathway regulated by MARCH1. CELL BIOLOGY INTERNATIONAL Cell Biol Int. 2021 Feb;45(2):368-381 WB Human Colorectal cancer(CRC ) tissues sw480s,DLD-1s,NCM460s

Dexamethasone Protects Against Ischaemic Brain Injury via Inhibiting the pAkt Signalling Pathway Through Increasing Hap1. NEUROTOXICITY RESEARCH 2020 Sep 02 WB Mouse 1 : 500 Brain

Anti-apoptotic and apoptotic pathway analysis of arsenic trioxide‑induced apoptosis in human gastric cancer SGC-7901 cells. ONCOLOGY REPORTS 2014 Jun 20 WB Human 1:800 SGC-7901 cell

Mn-TAT PTD-Ngb attenuates oxidative injury by an enhanced ROS scavenging ability and the regulation of redox signaling pathway. Scientific Reports Sci Rep-Uk. 2019 Dec;9(1):1-12 WB Rat 1:1000 PC12 cell

MicroRNA-7-5p regulates human alveolar epithelial sodium channels by targeting the mTORC2/SGK-1 signaling pathway. EXPERIMENTAL LUNG RESEARCH 2016 Jun 22 WB Human 1:1000 A549 cell

HSP90 inhibitor AUY922 can reverse Fulvestrant induced feedback reaction in human breast cancer cells. CANCER SCIENCE 2017 May 19 WB Human MCF-7 cell，T47D cell

Polygonum cuspidatum Extract Exerts Antihyperlipidemic Effects by Regulation of PI3K/AKT/FOXO3 Signaling Pathway. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2021;2021:3830671 IF,WB Human 1:1000 HepG2 cells

Hydroxy-α-sanshool Possesses Protective Potentials on H2O2-Stimulated PC12 Cells by Suppression of Oxidative Stress-Induced Apoptosis through Regulation of PI3K/Akt Signal Pathway. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2020;2020:3481758 WB Rat 1 : 1000 PC12 cell

Autophagy was activated against the damages of placentas caused by nano-copper oral exposure. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2021 Sep;220:112364 WB Rat 1 : 1000 Placentas

Intramuscular Delivery of scAAV9-hIGF1 Prolongs Survival in the hSOD1 G93A ALS Mouse Model via Upregulation of D-Amino Acid Oxidase. MOLECULAR NEUROBIOLOGY Mol Neurobiol. 2018 Jan;55(1):682-695 WB Mouse 1:1000 lumbar spinal cord tissue

PRMT5 promotes epithelial‐mesenchymal transition via EGFR‐β‐catenin axis in pancreatic cancer cells. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 2019 Dec 18 WB Human PaTu8988 cell, SW1990 cell

Cadmium induces BNIP3-dependent autophagy in chicken spleen by modulating miR-33-AMPK axis. CHEMOSPHERE 2017 Dec 06 WB Chicken 1:500 Spleen

The PI3K/p-Akt signaling pathway participates in calcitriol ameliorating podocyte injury in DN rats. METABOLISM-CLINICAL AND EXPERIMENTAL Metabolism. 2014 Oct;63:1324 WB Rat Renal tissues

Effects of enriched environment on depression and anxiety-like behavior induced by early life stress: A comparison between different periods. BEHAVIOURAL BRAIN RESEARCH Behav Brain Res. 2021 Aug;411:113389 WB Mouse 1 : 1000 Hippocampal tissue

MicroRNA-32 promotes calcification in vascular smooth muscle cells: Implications as a novel marker for coronary artery calcification. PLoS One Plos One. 2017 Mar;12(3):e0174138 WB Mouse MVSMCs

An Integrated Approach Based on Network Pharmacology Combined with Experimental Verification Reveals AMPK/PI3K/Akt Signaling is an Important Way for the Anti-Type 2 Diabetic Activity of Silkworm Excrement. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy Diabet Metab Synd Ob. 2021 Feb;14:601-616 WB Human 1:1000 HepG2 cell

Silver Nanoparticles Induced Oxidative Stress and Mitochondrial Injuries Mediated Autophagy in HC11 Cells Through Akt/AMPK/mTOR Pathway. BIOLOGICAL TRACE ELEMENT RESEARCH Biol Trace Elem Res. 2021 Mar;199(3):1062-1073 WB Human 1:1000 HC11 cell

miR‐134 inhibits non‐small cell lung cancer growth by targeting the epidermal growth factor receptor. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE J Cell Mol Med. 2016 Oct;20(10):1974-1983 WB Human 1:1000 A549 cell, H1299 cell, H520 cell,H1975 cell

Gastrointestinal Factor GDDR Attenuates Epithelial–Mesenchymal Transition in Gastric Cancer via Inhibiting AKT Signal. DIGESTIVE DISEASES AND SCIENCES Digest Dis Sci. 2016 Jul;61(7):1941-1949 WB Human gastric cancer tissues SGC7901 cell

CCL21/CCR7 interaction promotes cellular migration and invasion via modulation of the MEK/ERK1/2 signaling pathway and correlates with lymphatic metastatic spread and poor prognosis in urinary bladder cancer. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2017 Jul;51(1):75-90 WB Human 1:2000 UM-UC-3 cell

Cardamonin inhibits angiotensin II-induced vascular smooth muscle cell proliferation and migration by downregulating p38 MAPK, Akt, and ERK phosphorylation. Journal of Natural Medicines J Nat Med-Tokyo. 2014 Jul;68(3):623-629 WB Rat VSMCs

PDGF-mediated PI3K/AKT/β-catenin signaling regulates gap junctions in corpus cavernosum smooth muscle cells. EXPERIMENTAL CELL RESEARCH Exp Cell Res. 2018 Jan;362:252 WB Rat 1:1000 corpus cavernosum smooth muscle cells (CCSMCs)

Induction of PI3K/Akt-Mediated Apoptosis in Osteoclasts Is a Key Approach for Buxue Tongluo Pills to Treat Osteonecrosis of the Femoral Head.. Frontiers in Pharmacology Front Pharmacol. 2021 Nov;12:729909-729909 WB Mouse 1:1000 RAW 264.7 cell

Negative Regulation of Grb10 Interacting GYF Protein 2 on Insulin-Like Growth Factor-1 Receptor Signaling Pathway Caused Diabetic Mice Cognitive Impairment. PLoS One 2014 Sep 30 WB Mouse 1∶1000 hippocampus

Effects of vitamin D and resveratrol on metabolic associated markers in liver and adipose tissue from SAMP8 mice. EXPERIMENTAL GERONTOLOGY 2017 Apr 12 WB Mouse liver, epididymal ,subcutaneous adipose tissue

Inactivation of the Phosphatidylinositol 3‐Kinase/Akt Pathway is Involved in BMP9‐mediated Tumor‐suppressive Effects in Gastric Cancer Cells. JOURNAL OF CELLULAR BIOCHEMISTRY 2015 Apr 10 WB,IHC Human 1:1000 gastric cancer tissue SGC-7901 cell, MNK-45 cell

FGF9 knockout in GABAergic neurons induces apoptosis and inflammation via the Fas/caspase-3 pathway in the cerebellum of mice. BRAIN RESEARCH BULLETIN 2019 Nov 11 WB Mouse tail tissue

CD90 affects the biological behavior and energy metabolism level of gastric cancer cells by targeting the PI3K/AKT/HIF‑1α signaling pathway. Oncology Letters Oncol Lett. 2021 Mar;21(3):1-1 WB Human 1:1000 AGS cell

FGF9 modulates Schwann cell myelination in developing nerves and induces a pro‐inflammatory environment during injury. JOURNAL OF CELLULAR BIOCHEMISTRY J Cell Biochem. 2018 Oct;119(10):8643-8658 WB Mouse sciatic nerves

scAAV9-VEGF prolongs the survival of transgenic ALS mice by promoting activation of M2 microglia and the PI3K/Akt pathway. BRAIN RESEARCH Brain Res. 2016 Oct;1648:1 WB Mouse 1:500 spinal

Intrathecal Delivery of ssAAV9-DAO Extends Survival in SOD1G93A ALS Mice. NEUROCHEMICAL RESEARCH Neurochem Res. 2017 Apr;42(4):986-996 WB Mouse spinal cord

Ad-p53 enhances the sensitivity of triple-negative breast cancer MDA-MB-468 cells to the EGFR inhibitor gefitinib. ONCOLOGY REPORTS 2014 Dec 11 WB Human MDA-MB-468 cell

AAV9-IGF1 protects TDP-25 cells from apoptosis and oxidative stress partly via up-regulating the expression of VEGF in vitro. NEUROSCIENCE LETTERS Neurosci Lett. 2017 Feb;640:123 WB Human 1:500 TDP-25 cell

Overexpression of ATG4a promotes autophagy and proliferation, and inhibits apoptosis in lens epithelial cells via the AMPK and Akt pathways. Molecular Medicine Reports Mol Med Rep. 2020 Aug;22(2):1295-1302 WB Human 1 : 1000 Lens epithelial cells

Scutellarin Prevents Nonalcoholic Fatty Liver Disease (NAFLD) and Hyperlipidemia via PI3K/AKT-Dependent Activation of Nuclear Factor (Erythroid-Derived 2)-Like 2 (Nrf2) in Rats. MEDICAL SCIENCE MONITOR Med Sci Monitor. 2017; 23: 5599–5612 WB,IF Rat 1: 400 liver tissue

Adropin reduces hypoxia/reoxygenation‑induced myocardial injury via the reperfusion injury salvage kinase pathway. Experimental and Therapeutic Medicine Exp Ther Med. 2019 Nov;18(5):3307-3314 WB Rat 1:1000 H9c2 cell

The effects of hyaluronic acid vaginal gel on the vaginal epithelium of ovariectomized rats. GYNECOLOGICAL ENDOCRINOLOGY Gynecol Endocrinol. 2015;31(3):208-213 WB Rat vaginal Cytoplasmic

Tian, Sha-Sha, et al. "Flavonoids from the leaves of Carya cathayensis Sarg. inhibit vascular endothelial growth factor-induced angiogenesis." Fitoterapia 92 (2014): 34-40.

Wang, Wei-wei, et al. "Telmisartan reduces atrial arrhythmia susceptibility through the regulation of RAS–ERK and PI3K–Akt–eNOS pathways in spontaneously hypertensive rats." Canadian journal of physiology and pharmacology 93.8 (2015): 657-665.

Cao, Zheng, et al. "Exendin-4 attenuates high glucose-induced endothelial progenitor cells dysfunction through the PI3-K/Akt/eNOS pathway." INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 10.6 (2017): 6774-6780.

Zhang, Xianxie, et al. "Salvia miltiorrhiza alleviates hypoxia-induced nerve injury associated with Ngb/Akt intracellular signaling pathway." (2020).

刘素娟, et al. "染料木素通过 TIPE 2 负性调控 Akt 活性促进脂多糖活化的巨噬细胞凋亡." (2020).

佟雪, et al. "基于 PI3K/AKT/mTOR 信号通路研究补肾法对体外培养小鼠卵母细胞成熟的促进作用." 北京中医药大学学报 44.6 (2021): 510-518.

恪雪, et al. "基于 PI3K/AKT/mTOR 信号通路研究补肾法对体外培养 小鼠卵母细胞成熟的促进作." Journal of Beijing University of Traditional Chinese Medicine 44.6 (2021).

Cadmium induces BNIP3-dependent autophagy in chicken spleen by modulating miR-33-AMPK axis. CHEMOSPHERE 2017 Dec 06 WB Chicken 1:1000 Spleen

5‐FU inhibits migration and invasion of CRC cells through PI3K/AKT pathway regulated by MARCH1. CELL BIOLOGY INTERNATIONAL Cell Biol Int. 2021 Feb;45(2):368-381 WB Human Colorectal cancer(CRC ) tissues sw480s,DLD-1s,NCM460s

Dexamethasone Protects Against Ischaemic Brain Injury via Inhibiting the pAkt Signalling Pathway Through Increasing Hap1. NEUROTOXICITY RESEARCH 2020 Sep 02 WB Mouse 1 : 500 Brain

Baicalein inhibits non‐small‐cell lung cancer invasion and metastasis by reducing ezrin tension in inflammation microenvironment. CANCER SCIENCE Cancer Sci. 2020 Oct; 111(10): 3802–3812 WB Human A549 cell， H1299 cell

COX-2/sEH Dual Inhibitor Alleviates Hepatocyte Senescence in NAFLD Mice by Restoring Autophagy through Sirt1/PI3K/AKT/mTOR INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Chen-Yu Zhang WB Mouse

The Hypoglycemic Effect of JinQi Jiangtang Tablets Is Partially Dependent on the Palmatine-Induced Activation of the Fibroblast Growth Factor Receptor 1 Signaling Pathway Frontiers in Pharmacology Yubin Ji WB Human

Arsenic Trioxide-loaded nanoparticles Enhance the Chemosensitivity of Gemcitabine in Pancreatic Cancer via Reversal of Pancreatic Stellate Cells Desmoplasia through Targeting AP4/Galectin-1 Pathway Biomaterials Science Kaihong Huang WB Human

6-Methoxydihydrosanguinarine induces apoptosis and autophagy in breast cancer MCF-7 cells by accumulating ROS to suppress the PI3K/AKT/mTOR signaling pathway PHYTOTHERAPY RESEARCH Hui Zhang WB Human

CB2R agonist GW405833 alleviates acute liver failure in mice via inhibiting HIF-1α-mediated reprogramming of glycometabolism and macrophage proliferation ACTA PHARMACOLOGICA SINICA Ze-bing Huang WB Mouse RAW264.7 cells

Sec61γ is a vital protein in the endoplasmic reticulum membrane promoting tumor metastasis and invasion in lung adenocarcinoma BRITISH JOURNAL OF CANCER Lishuang Qi WB Human,Mouse lung cancer A549 cell,H1650 cell

Anti-Inflammatory Effects of Allocryptopine via the Target on the CX3CL1–CX3CR1 axis/GNB5/AKT/NF-κB/Apoptosis in Dextran Sulfate-Induced Mice Biomedicines Yisong Liu WB Mouse Colon tissue

HMGB1 released from dead tumor cells after insufficient radiofrequency ablation promotes progression of HCC residual tumor via ERK1/2 pathway INTERNATIONAL JOURNAL OF HYPERTHERMIA Baoming Luo WB Human HepG2 cell,Huh7 cell

UQCRFS1 serves as a prognostic biomarker and promotes the progression of ovarian cancer. Jing Wang WB Human 1:1000 ovarian tissue A2780 cell,OVCAR8 cell

Hepatoprotective efficacy and interventional mechanism of JianPi LiShi YangGan formula in acute-on-chronic liver failure. Xiaozhou Zhou IHC，WB Mouse 1:200，1:1000 liver AML12 cell

FGF12 regulates cell cycle gene expression and promotes follicular granulosa cell proliferation through ERK phosphorylation in geese. Xingyong Chen WB Geese 1:1000 granulosa cell

Upregulation of Pin1 contributes to alleviation of cognitive dysfunction in diabetic mice Brain and Behavior Dan Qiu WB Mouse 1:800 hippocampus

Study of the Mechanism of Astragali Radix in Treating Type 2 Diabetes Mellitus and Its Renal Protection Based on Enzyme Activity, Network Pharmacology, and Experimental Verification MOLECULES Chunnan Li WB Mouse renal tissue

CXADR promote epithelial–mesenchymal transition in endometriosis by modulating AKT/GSK-3β signaling CELL CYCLE Hang-Jing Tan WB Human 1:1000 Ishikawa cell

Roflupram alleviates autophagy defects and reduces mutant hSOD1-induced motor neuron damage in cell and mouse models of amyotrophic lateral sclerosis NEUROPHARMACOLOGY Di Huo WB Mouse 1:1000 spinal cord NSC34 cell

Inhibitor PF-04691502 works as a senolytic to regulate cellular senescence EXPERIMENTAL GERONTOLOGY Ziqiang Fan WB Mouse 1:500 NIH-3T3 cell

The gut microbiota contributes to the infection of bovine viral diarrhea virus in mice JOURNAL OF VIROLOGY Zecai Zhang WB Mouse peripheral blood lymphocytes (PBL)

Ganoderic acids alleviate atherosclerosis by inhibiting macrophage M1 polarization via TLR4/MyD88/NF-κB signaling pathway ATHEROSCLEROSIS Ya-zhu Quan WB Mouse bone marrow dereived macrophages (BMDMs)

MiR-21-5p ameliorates Busulfan-induced testicular dysfunction and maintains spermatogenesis1 Journal of Integrative Agriculture Meng-fei ZHANG WB Goat Male germ stem cells (mGSCs)

Integrin β4 Regulates Cell Migration of Lung Adenocarcinoma Through FAK Signaling MOLECULAR BIOTECHNOLOGY Zhang Shusen WB Human 1:1000 PC9 cell,NCIH1975 cell,A549 cell

NUP85 alleviates lipid metabolism and inflammation by regulating PI3K/AKT signaling pathway in nonalcoholic fatty liver disease International Journal of Biological Sciences Wu Yin-cui WB Mouse AML-12 cell

Antidepressant effects of esketamine via the BDNF/AKT/mTOR pathway in mice with postpartum depression and their offspring PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY Han Qin WB Mouse 1:1000 frontal lobe tissue,hippocampus tissue

Rutin targets AKT to inhibit ferroptosis in ventilator-induced lung injury PHYTOTHERAPY RESEARCH Shengsong Chen IHC,WB Mouse Lung tissue

The FGF6 amplification mutation plays an important role in the progression and treatment of malignant meningioma Translational Oncology Ruihao Liang WB Human IOMM-LEE cell,CH157MN cell

Dexmedetomidine improves the circulatory dysfunction of the glymphatic system induced by sevoflurane through the PI3K/AKT/ΔFosB/AQP4 pathway in young mice Cell Death & Disease Wang Shuying WB Mouse 1:2000 hippocampus tissue primary astrocytes

Naringenin modulates the NO‑cGMP‑PKG signaling pathway by binding to AKT to enhance osteogenic differentiation in hPDLSCs INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Shenghong Li WB Human 1:2000 human periodontal ligament stem cell(hPDLSC)

Promoter hypermethylation-induced downregulation of ITGA7 promotes colorectal cancer proliferation and migration by activating the PI3K/AKT/NF-κB pathway BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH Jianjun Wang WB Human 1:1000 SW480 cell,SW620 cell

Icariin, a natural flavonoid glucoside, inhibits neuroinflammation in mice with triple-transgenic Alzheimer’s disease by regulating the Akt/GSK-3β signaling pathway Journal of Functional Foods Sichen Wu WB Mouse cerebral cortex tissue

Neuroprotective effects of total phenolics from Hemerocallis citrina Baroni leaves through the PI3K/AKT pathway Frontiers in Pharmacology Jia Yanjun WB Rat PC12 cell

miR-107 reverses the multidrug resistance of gastric cancer by targeting the CGA/EGFR/GATA2 positive feedback circuit JOURNAL OF BIOLOGICAL CHEMISTRY Pei Wang IHC Mouse 1:200 SGC7901 cell-Xenograft

Kaempferol inhibited invasion and metastasis of gastric cancer cells by targeting AKT/GSK3β pathway based on network pharmacology and molecular docking JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH Xia-Qing Gao WB Human 1:1000 HGC27 cell,MKN45 cell

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