• Target：
• NOS3

• Fields：
• >>Arginine biosynthesis;>>Arginine and proline metabolism;>>Metabolic pathways;>>Calcium signaling pathway;>>cGMP-PKG signaling pathway;>>HIF-1 signaling pathway;>>Sphingolipid signaling pathway;>>PI3K-Akt signaling pathway;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Platelet activation;>>Estrogen signaling pathway;>>Oxytocin signaling pathway;>>Relaxin signaling pathway;>>Insulin resistance;>>AGE-RAGE signaling pathway in diabetic complications;>>Diabetic cardiomyopathy;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis

• Gene Name：
• NOS3

• Protein Name：
• Nitric oxide synthase, endothelial

• Human Gene Id：
• 4846

• Human Swiss Prot No：
• P29474

• Mouse Gene Id：
• 18127

• Mouse Swiss Prot No：
• P70313

• Rat Gene Id：
• 24600

• Rat Swiss Prot No：
• Q62600

• Specificity：
• endogenous

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Monoclonal, rabbit, IgG, Kappa

• Dilution：
• IHC 1:1000-1:4000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;

• Purification：
• Protein A

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NOS3;Nitric oxide synthase, endothelial;Constitutive NOS;cNOS;EC-NOS;Endothelial NOS;eNOS;NOS type III;NOSIII

• Molecular Weight(Da)：
• 133kD

• Observed Band(KD)：
• 133kD

• Background：
• Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],

• Function：
• catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.,function:Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.,online information:Nitric oxide synthase entry,polymorphism:Variation in NOS3 seem to be associated with susceptibility to coronary spasm.,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like

• Subcellular Location：
• Membrane

• Expression：
• Platelets, placenta, liver and kidney.

SIRT3-AMPK signaling pathway as a protective target in endothelial dysfunction of early sepsis Int Immunopharmacol. 2022 May;106:108600. WB Human,Mouse 1:1000 aorta

PM2.5 induces autophagy-mediated cell death via NOS2 signaling in human bronchial epithelium cells. International Journal of Biological Sciences Int J Biol Sci. 2018; 14(5): 557–564 WB Human BEASE-2B cell

Lu, Qian, et al. "Norisoboldine suppresses VEGF-induced endothelial cell migration via the cAMP-PKA-NF-κB/Notch1 pathway." PloS one 8.12 (2013): e81220.

Limosilactobacillus reuteri ameliorates preeclampsia in mice via improving gut dysbiosis and endothelial dysfunction BIOMEDICINE & PHARMACOTHERAPY Dongxin Zhang WB Mouse placenta HUVECs

Naringenin modulates the NO‑cGMP‑PKG signaling pathway by binding to AKT to enhance osteogenic differentiation in hPDLSCs INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Shenghong Li WB Human 1:2000 human periodontal ligament stem cell(hPDLSC)

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