• Target：
• HSP A5/GRP78

• Fields：
• >>Protein export;>>Protein processing in endoplasmic reticulum;>>Antigen processing and presentation;>>Thyroid hormone synthesis;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Lipid and atherosclerosis

• Gene Name：
• HSPA5 GRP78

• Protein Name：
• GRP78 BiP

• Human Gene Id：
• 3309

• Human Swiss Prot No：
• P11021

• Mouse Gene Id：
• 14828

• Mouse Swiss Prot No：
• P20029

• Rat Swiss Prot No：
• P06761

• Specificity：
• endogenous

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Monoclonal, rabbit, IgG, Kappa

• Dilution：
• IHC 1:200-1:1000,WB 1:1000-1:5000,IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200,

• Purification：
• Protein A

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• 78 kDa glucose-regulated protein (GRP-78) (Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78) (Heat shock 70 kDa protein 5) (Immunoglobulin heavy chain-binding protein) (BiP)

• Molecular Weight(Da)：
• 72kD

• Observed Band(KD)：
• 78kD

• Background：
• The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010],

• Function：
• disease:Autoantigen in rheumatoid arthritis [MIM:180300].,function:Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.,similarity:Belongs to the heat shock protein 70 family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Interacts with DNAJC1 (via J domain) (By similarity). Component of an EIF2 complex at least composed of CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising CABP1, DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with TMEM132A.,

• Subcellular Location：
• Cytoplasm

• Expression：
• Articular cartilage,Brain,Cajal-Retzius cell,Cervix carcino

Chronic hypoxia and Cu2+ exposure induce gill remodeling of Largemouth bass through endoplasmic reticulum stress, mitochondrial damage and apoptosis AQUATIC TOXICOLOGY Qiao Liu, Hong Wang, Jiayu Ge, Lisen Li, Jie Luo, Kuo He, Haoxiao Yan, Xin Zhang, Rabia Tahir, Wei Luo, Shiyi Chen, Zhang Cheng, Liulan Zhao, Song Yang WB Fish Gill tissue

Overexpression of long non‐coding RNA NORAD promotes invasion and migration in malignant melanoma via regulating the MIR‐205‐EGLN2 pathway. Cancer Medicine Cancer Med-Us. 2019 Apr;8(4):1744-1754 WB Human A375 cell, WM35 cell

Protective effects of circulating microvesicles derived from ischemic preconditioning on myocardial ischemia/reperfusion injury in rats by inhibiting endoplasmic reticulum stress. APOPTOSIS Apoptosis. 2018 Aug;23(7):436-448 IHC Rat left ventricle

The combination of 1α,25dihydroxyvitaminD3 with resveratrol improves neuronal degeneration by regulating endoplasmic reticulum stress, insulin signaling and inhibiting tau hyperphosphorylation in SH-SY5Y cells. FOOD AND CHEMICAL TOXICOLOGY 2016 Apr 28 WB Human 1:1000 SH-SY5Y total cell

Endoplasmic reticulum stress contributes to NMDA-induced pancreatic β-cell dysfunction in a CHOP-dependent manner. LIFE SCIENCES 2019 Jun 28 WB Mouse 1:1000 MIN6 cell

Effects of vitamin D and resveratrol on metabolic associated markers in liver and adipose tissue from SAMP8 mice. EXPERIMENTAL GERONTOLOGY 2017 Apr 12 WB Mouse liver, epididymal ,subcutaneous adipose tissue

Sodium fluoride induces apoptosis through reactive oxygen species-mediated endoplasmic reticulum stress pathway in Sertoli cells. Journal of Environmental Sciences 2015 Feb 24 WB Rat Sertoli cell

Thymosin β4 prevents oxygen-glucose deprivation/reperfusion-induced injury in rat cortical neurons. Neuropsychiatric Disease and Treatment Neuropsych Dis Treat. 2019; 15: 2385–2393 WB Rat 1:1000 cortical neurons

Overexpression of thioredoxin reductase 1 can reduce DNA damage, mitochondrial autophagy and endoplasmic reticulum stress in Parkinson's disease. EXPERIMENTAL BRAIN RESEARCH Exp Brain Res. 2021 Feb;239(2):475-490 IHC Mouse Brain

Yang, Jie, et al. "Thioredoxin silencing-induced cardiac supercontraction occurs through endoplasmic reticulum stress and calcium overload in chicken." Metallomics 10.11 (2018): 1667-1677.

The combination of 1α, 25dihydroxyvitaminD3 with resveratrol improves neuronal degeneration by regulating endoplasmic reticulum stress, insulin signaling and inhibiting tau hyperphosphorylation in SH-SY5Y cells." Food and Chemical Toxicology 93 (2016): 32-40.

Cheng, Jinbo, et al. "Vitamin D combined with resveratrol prevents cognitive decline in SAMP8 mice." Current Alzheimer Research 14.8 (2017): 820-833.

Cadmium induces liver dysfunction and ferroptosis through the endoplasmic stress-ferritinophagy axis ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Jiacheng Wu WB Mouse

Physiological responses to acute hypoxia in the liver of largemouth bass by alteration of mitochondrial function and Ca2+ exchange AQUATIC TOXICOLOGY Song Yang WB Fish Liver tissue

Disturbance of neuron–microglia crosstalk mediated by GRP78 in Neuropsychiatric systemic lupus erythematosus mice. Ling Qin IHC,IF Mouse 1:200 brain tissue BV2 cell

α-Hederin induces human colorectal cancer cells apoptosis through disturbing protein homeostasis. CHEMICO-BIOLOGICAL INTERACTIONS Haibo Cheng WB Human HCT116 cell,SW480 cell

Cepharanthine triggers ferroptosis through inhibition of NRF2 for robust ER stress against lung cancer EUROPEAN JOURNAL OF PHARMACOLOGY Xiao-Feng Bai WB Mouse,Human Lewis cell-Xenograft A549 cell,Lewis cell

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