• Gene Name：
• >>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>Sphingolipid signaling pathway;>>Vascular smooth muscle contraction;>>TGF-beta signaling pathway;>>Axon guidance;>>Focal adhesion;>>Tight junction;>>Platelet activation;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton;>>Oxytocin signaling pathway;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Yersinia infection;>>Human cytomegalovirus infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>MicroRNAs in cancer

• Protein Name：
• ROCK1

• Sequence：
• Rho-associated protein kinase 1

• Human Gene Id：
• 6093

• Human Swiss Prot No：
• Q13464

• Mouse Gene Id：
• 19877

• Mouse Swiss Prot No：
• P70335

• Rat Gene Id：
• 81762

• Rat Swiss Prot No：
• Q63644

• Specificity：
• endogenous

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Monoclonal, rabbit, IgG, Kappa

• Dilution：
• IHC 1:200-1:1000,WB 1:1000-1:5000,IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200,

• Purification：
• Protein A

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ROCK1;Rho-associated protein kinase 1;Renal carcinoma antigen NY-REN-35;Rho-associated; coiled-coil-containing protein kinase 1;Rho-associated, coiled-coil-containing protein kinase I;ROCK-I;p160 ROCK-1;p160ROCK

• Molecular Weight(Da)：
• 158kD

• Observed Band(KD)：
• 158kD

• Background：
• This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.,enzyme regulation:Activated by RHOA binding.,function:Protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Necessary for apoptotic membrane blebbing. Plays a role in smooth muscle contraction. Required for centromere positioning and centromere-dependent exit from mitosis.,miscellaneous:Inhibited by Y-27632.,PTM:Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Cleaved by caspase-3 during ap

• Subcellular Location：
• Cytoplasm, Membrane

• Expression：
• Detected in blood platelets.

Profilin 1 Induces Tumor Metastasis by Promoting Microvesicle Secretion Through the ROCK 1/p-MLC Pathway in Non-Small Cell Lung Cancer IF Human 1:100 non-small cell lung cancer (NSCLC) tissues/

Modulation of SEMA4D-modified titanium surface on M2 macrophage polarization via activation of Rho/ROCK-mediated lactate release of endothelial cells: In vitro and in vivo COLLOIDS AND SURFACES B-BIOINTERFACES Jie-yi Zhou IF Mouse 1:100 bEnd.3 cell

Exercise Attenuate Diaphragm Atrophy in COPD Mice via Inhibiting the RhoA/ROCK Signaling International Journal of Chronic Obstructive Pulmonary Disease Peijun Li WB Mouse 1:700 diaphragm tissue C2C12 cell

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