• Target：
• CREB-2

• Fields：
• >>MAPK signaling pathway;>>cGMP-PKG signaling pathway;>>Mitophagy - animal;>>Protein processing in endoplasmic reticulum;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Longevity regulating pathway;>>Adrenergic signaling in cardiomyocytes;>>TNF signaling pathway;>>Long-term potentiation;>>Neurotrophin signaling pathway;>>Cholinergic synapse;>>Dopaminergic synapse;>>Insulin secretion;>>GnRH signaling pathway;>>Estrogen signaling pathway;>>Thyroid hormone synthesis;>>Glucagon signaling pathway;>>Aldosterone synthesis and secretion;>>Relaxin signaling pathway;>>Cortisol synthesis and secretion;>>Parathyroid hormone synthesis, secretion and action;>>Non-alcoholic fatty liver disease;>>Cushing syndrome;>>Growth hormone synthesis, secretion and action;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Cocaine addiction;>>Amphetamine addiction;>>Alcoholism;>>Hepatitis B;>>Human cytomegalovirus infection;>>Hu

• Gene Name：
• ATF4

• Protein Name：
• Cyclic AMP-dependent transcription factor ATF-4

• Human Gene Id：
• 468

• Human Swiss Prot No：
• P18848

• Mouse Gene Id：
• 11911

• Mouse Swiss Prot No：
• Q06507

• Specificity：
• endogenous

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Monoclonal, rabbit, IgG, Kappa

• Dilution：
• IHC 1:100-1:200,WB 1:1000-1:5000,IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200,

• Purification：
• Protein A

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ATF4;CREB2;TXREB;Cyclic AMP-dependent transcription factor ATF-4;cAMP-dependent transcription factor ATF-4;Activating transcription factor 4;Cyclic AMP-responsive element-binding protein 2;CREB-2;cAMP-responsive element-binding prot

• Molecular Weight(Da)：
• 38kD

• Observed Band(KD)：
• 49kD

• Background：
• activating transcription factor 4(ATF4) Homo sapiens This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provid

• Function：
• function:Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.,similarity:Belongs to the bZIP family.,similarity:Contains 1 bZIP domain.,subcellular location:Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.,subunit:Interacts with the C-terminal region of GABBR1 via the leucine zipper of its C-terminal bZIP domain. Interacts with the C-terminal region of GABBR2 (By similarity). Binds DNA as a homo-or heterodimer. Interacts with the N-terminal region of CEP290.,

• Subcellular Location：
• Nucleus

• Expression：
• Bladder,Colon,Fibroblast,Leukemic T-cell,Lung,Ovary,Placenta,

Huaier Induces Immunogenic Cell Death Via CircCLASP1/PKR/eIF2α Signaling Pathway in Triple Negative Breast Cancer WB Human /MDA-MB-231 cells

Huaier Induces Immunogenic Cell Death Via CircCLASP1/PKR/eIF2α Signaling Pathway in Triple Negative Breast Cancer WB Human /MDA-MB-231 cells

Classical Swine Fever Virus Infection Induces Endoplasmic Reticulum Stress-Mediated Autophagy to Sustain Viral Replication in vivo and in vitro. Frontiers in Microbiology Front Microbiol. 2019 Nov;0:2545 WB Pig tonsil, spleen, thymus, lymph nodes,lung, kidney, intestine

CSFV Infection Up-Regulates the Unfolded Protein Response to Promote Its Replication. Frontiers in Microbiology Front Microbiol. 2017 Nov;8:2129 WB Pig PK-15 cell

Chlamydia trachomatis plasmid-encoded protein pORF5 activates unfolded protein response to induce autophagy via MAPK/ERK signaling pathway. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2020 May 20 WB Human HeLa cell

PERK signalling pathway mediates single prolonged stress-induced dysfunction of medial prefrontal cortex neurons. APOPTOSIS Apoptosis. 2017 Jun;22(6):753-768 WB Rat 1:500 neurons

Cadmium induces liver dysfunction and ferroptosis through the endoplasmic stress-ferritinophagy axis ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Jiacheng Wu WB Mouse

Shikonin potentiates therapeutic efficacy of oxaliplatin through reactive oxygen species-mediated intrinsic apoptosis and endoplasmic reticulum stress in oxaliplatin-resistant colorectal cancer cells DRUG DEVELOPMENT RESEARCH Yajie Zhang WB Human,Mouse HCT116R-xenograft OXA-resistant CRC cells (HCT116R)

Ferroptosis is involved in Staphylococcus aureus-induced mastitis through autophagy activation by endoplasmic reticulum stress INTERNATIONAL IMMUNOPHARMACOLOGY Lijuan Bao WB Mouse 1:1000 Mouse mammary epithelial cell(MMEC)

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs