• Target：
• LC3B

• Fields：
• >>Mitophagy - animal;>>Autophagy - animal;>>Ferroptosis;>>Apelin signaling pathway;>>NOD-like receptor signaling pathway;>>Amyotrophic lateral sclerosis;>>Pathways of neurodegeneration - multiple diseases;>>Shigellosis;>>Kaposi sarcoma-associated herpesvirus infection

• Gene Name：
• MAP1LC3B

• Protein Name：
• MAP1LC3B

• Human Gene Id：
• 81631

• Human Swiss Prot No：
• Q9GZQ8

• Mouse Swiss Prot No：
• Q9CQV6

• Specificity：
• endogenous

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Monoclonal, rabbit, IgG, Kappa

• Dilution：
• IHC 1:200-1:1000,WB 1:1000-1:5000,IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200,

• Purification：
• Protein A

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MAP1LC3B

• Molecular Weight(Da)：
• 14kD,16kD

• Observed Band(KD)：
• 14kD,16kD

• Background：
• The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008],

• Function：
• caution:PubMed:12740394 has shown that the protein is cleaved at Lys-122 but PubMed:15355958 has shown that the cleavage site is at Gly-120 as in other mammalian orthologs.,function:Probably involved in formation of autophagosomal vacuoles (autophagosomes).,PTM:The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.,similarity:Belongs to the MAP1 LC3 family.,subcellular location:LC3-II binds to the autophagic membranes.,subunit:3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.,tissue specificity:Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.,

• Subcellular Location：
• Cytoplasm

• Expression：
• Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.

Outer membrane protein a in Acinetobacter baumannii induces autophagy through mTOR signalling pathways in the lung of SD rats. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2021 Mar;135:111034 IF,IHC,WB Rat 1:100,1: 500,1:100 Lung WTRL1 cell

Ammonia inhalation-mediated mir-202-5p leads to cardiac autophagy through PTEN/AKT/mTOR pathway. CHEMOSPHERE Chemosphere. 2019 Nov;235:858 WB Human 1:500 heart

Dynamic Expression of HDAC3 in db/db Mouse RGCs and Its Relationship with Apoptosis and Autophagy. Journal of Diabetes Research J Diabetes Res. 2020;2020:6086780 IHC Mouse 1 : 100 Retinal tissues

Quantitative Proteomic Analysis Reveals That Arctigenin Alleviates Concanavalin A-Induced Hepatitis Through Suppressing Immune System and Regulating Autophagy. Frontiers in Immunology 2018 Aug 16 WB,IHC Mouse liver

Verbascoside inhibits progression of glioblastoma cells by promoting Let‐7g‐5p and down‐regulating HMGA2 via Wnt/beta‐catenin signalling blockade. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE J Cell Mol Med. 2020 Mar;24(5):2901-2916 IF Human 1:200 U87 cell,U251 cell

Quantitative Proteomic Analysis Reveals That Arctigenin Alleviates Concanavalin A-Induced Hepatitis Through Suppressing Immune System and Regulating Autophagy. Frontiers in Immunology 2018 Aug 16 WB Mouse liver

Verbascoside inhibits progression of glioblastoma cells by promoting Let‐7g‐5p and down‐regulating HMGA2 via Wnt/beta‐catenin signalling blockade. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE J Cell Mol Med. 2020 Mar;24(5):2901-2916 IF Human 1:200 U87 cell,U251 cell

Quantitative Proteomic Analysis Reveals That Arctigenin Alleviates Concanavalin A-Induced Hepatitis Through Suppressing Immune System and Regulating Autophagy. Frontiers in Immunology 2018 Aug 16 WB Mouse liver

Verbascoside inhibits progression of glioblastoma cells by promoting Let‐7g‐5p and down‐regulating HMGA2 via Wnt/beta‐catenin signalling blockade. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE J Cell Mol Med. 2020 Mar;24(5):2901-2916 IF Human 1:200 U87 cell,U251 cell

The natural medicinal fungus Huaier promotes the anti-hepatoma efficacy of sorafenib through the mammalian target of rapamycin-mediated autophagic cell death MEDICAL ONCOLOGY Zhengguang Zhang WB Mouse, Human

Hypoxia Aggravates Neuron Ferroptosis in Early Brain Injury Following Subarachnoid Hemorrhage via NCOA4-Meditated Ferritinophagy Antioxidants Zixuan Yuan WB Mouse 1:1000 brain tissue HT22 cell

Tetrahedral framework nucleic acids-based delivery of microRNA-155 alleviates intervertebral disc degeneration through targeting Bcl-2/Bax apoptosis pathway CELL PROLIFERATION Zhuhai Li IF Rat 1:200 Nucleus pulposus cell

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs