• Target：
• CHOP

• Fields：
• >>MAPK signaling pathway;>>Protein processing in endoplasmic reticulum;>>Apoptosis;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Transcriptional misregulation in cancer;>>Lipid and atherosclerosis

• Gene Name：
• DDIT3

• Protein Name：
• DDIT3

• Human Gene Id：
• 1649

• Human Swiss Prot No：
• P35638

• Mouse Swiss Prot No：
• P35639

• Rat Swiss Prot No：
• Q62857

• Specificity：
• endogenous

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Monoclonal, rabbit, IgG, Kappa

• Dilution：
• IHC 1:1000-1:4000;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;

• Purification：
• Protein A

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• DDIT3

• Molecular Weight(Da)：
• 19kD

• Observed Band(KD)：
• 30kD

• Background：
• This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010],

• Function：
• disease:A chromosomal aberration involving DDIT3 is found in a form of malignant myxoid liposarcoma [MIM:126337]. Translocation t(12;16)(q13;p11) with FUS.,function:Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA.,similarity:Belongs to the bZIP family.,similarity:Contains 1 bZIP domain.,subunit:Heterodimer.,

• Subcellular Location：
• Cytoplasm, Nucleus

• Expression：
• Muscle,Skeletal muscle,

Perilipin5 protects against lipotoxicity and alleviates endoplasmic reticulum stress in pancreatic β-cells. Nutrition & Metabolism 2019 Jul 30 WB Rat INS-1 cell

The Regulatory Effect of VEGF-Ax on Rat Bone Marrow Mesenchymal Stem Cells' Angioblastic Differentiation and Its Proangiogenic Ability. STEM CELLS AND DEVELOPMENT Stem Cells Dev. 2020 May;29(10):667-677 WB Rat 1:3000 Bone marrow mesenchymal stem cells(BMMSCs )

Isorhamnetin protects against bleomycin-induced pulmonary fibrosis by inhibiting endoplasmic reticulum stress and epithelial-mesenchymal transition. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 2019 Jan 01 WB Human,Mouse lung A549 cell,HBECs

Overexpression of long non‐coding RNA NORAD promotes invasion and migration in malignant melanoma via regulating the MIR‐205‐EGLN2 pathway. Cancer Medicine Cancer Med-Us. 2019 Apr;8(4):1744-1754 WB Human A375 cell, WM35 cell

Endogenous hydrogen sulfide alleviates methotrexate‐induced cognitive impairment by attenuating endoplasmic reticulum stress‐induced apoptosis via CHOP and caspase‐12. FUNDAMENTAL & CLINICAL PHARMACOLOGY Fund Clin Pharmacol. 2020 Oct;34(5):559-570 IHC Rat 1 : 100 hippocampus

Sodium fluoride induces apoptosis through reactive oxygen species-mediated endoplasmic reticulum stress pathway in Sertoli cells. Journal of Environmental Sciences 2015 Feb 24 WB Rat Sertoli cell

Endoplasmic reticulum stress plays an important role in methotrexate-related cognitive impairment in adult rats. International Journal of Clinical and Experimental Pathology Int J Clin Exp Patho. 2017; 10(10): 10252–10260 IF Rat 1:100 hippocampal tissues

Mitochondrial pathway and endoplasmic reticulum stress participate in the photosensitizing effectiveness of AE‐PDT in MG63 cells. Cancer Medicine 2016 Oct 03 WB Human MG63 cell

Liraglutide protects pancreatic β-cells against free fatty acids in vitro and affects glucolipid metabolism in apolipoprotein E‑/‑ mice by activating autophagy. Molecular Medicine Reports 2015 Jun 16 WB Rat INS-1 cell

Ko, Su Hyuk, et al. "Bacteroides fragilis Enterotoxin Induces the Formation of Autophagosomes in Endothelial Cells but Interferes Their Autophagosomal Fusion with Lysosomes for Complete Autophagic Flux Through a Mitogen-Activated Protein Kinase, AP-1, and C/EBP Homologous Protein-Dependent Pathway." Infection and immunity (2017): IAI-00420.

Mitochondrial pathway and endoplasmic reticulum stress participate in the photosensitizing effectiveness of AE‐PDT in MG63 cells. Cancer Medicine 2016 Oct 03 WB Human MG63 cell

Cadmium induces liver dysfunction and ferroptosis through the endoplasmic stress-ferritinophagy axis ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Jiacheng Wu WB Mouse

In Situ Vaccination with Mitochondria-Targeting Immunogenic Death Inducer Elicits CD8+ T Cell-Dependent Antitumor Immunity to Boost Tumor Immunotherapy. Ahu Yuan WB Mouse 4T1 cell

α-Hederin induces human colorectal cancer cells apoptosis through disturbing protein homeostasis. CHEMICO-BIOLOGICAL INTERACTIONS Haibo Cheng WB Human HCT116 cell,SW480 cell

Nano‑selenium alleviates the pyroptosis of cardiovascular endothelial cells in chicken induced by decabromodiphenyl ether through ERS-TXNIP-NLRP3 pathway SCIENCE OF THE TOTAL ENVIRONMENT Yangyang Jiang WB Chicken 1:8000 cardiovascular tissue Chicken arterial endothelial cells (PAECs)

Apigenin improves testosterone synthesis by regulating endoplasmic reticulum stress BIOMEDICINE & PHARMACOTHERAPY Liling Wu WB,ChIP Mouse testicular tissue TM3 cell

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs