• Target：
• NLRP3

• Fields：
• >>Necroptosis;>>NOD-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Pertussis;>>Yersinia infection;>>Influenza A;>>Coronavirus disease - COVID-19;>>Lipid and atherosclerosis

• Gene Name：
• NLRP3

• Protein Name：
• NACHT LRR and PYD domains-containing protein 3

• Human Gene Id：
• 114548

• Human Swiss Prot No：
• Q96P20

• Mouse Swiss Prot No：
• Q8R4B8

• Specificity：
• endogenous

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Monoclonal, rabbit, IgG, Kappa

• Dilution：
• IHC 1:200-1000,WB 1:500-5000,IF 1:200-1000,ELISA 1:5000-20000,IP 1:50-200

• Purification：
• Protein A

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NLRP3;C1orf7;CIAS1;NALP3;PYPAF1;NACHT, LRR and PYD domains-containing protein 3;Angiotensin/vasopressin receptor AII/AVP-like;Caterpiller protein 1.1CLR1.1;Cold autoinflammatory syndrome 1 protein;Cryopyrin;PYRIN-containing APAF1-like protein 1

• Observed Band(KD)：
• 115kD

• Background：
• This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are s

• Function：
• disease:Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.,disease:Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as 'neonatal onset multisystem inflammatory disease,' or NOMID, a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis, and joint manifestations with recurrent fever and inflammation.,disease:Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; commonly known as fami

• Subcellular Location：
• Cytoplasm, Nuclear

Crassifoside H ameliorates depressant behavior in chronic unpredictable mild stress rats by improving HPA axis dysfunction and inhibiting inflammation in hippocampus. TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH Trop J Pharm Res. 2020 Nov;19(8):1693-1699 WB 1 : 1000 Hippocampus sample

Gynostemma pentaphyllum polysaccharides ameliorate non-alcoholic steatohepatitis in mice associated with gut microbiota and the TLR2/NLRP3 pathway Frontiers in Endocrinology Rui-Rui Wang IHC Mouse

Gut-Flora-Dependent Metabolite Trimethylamine-N-Oxide Promotes Atherosclerosis-Associated Inflammation Responses by Indirect ROS Stimulation and Signaling Involving AMPK and SIRT1 Nutrients Wenjian Ma WB Human

IL-37 alleviates TNF-α-induced pyroptosis of rheumatoid arthritis fibroblast-like synoviocytes by inhibiting the NF-κB/GSDMD signaling pathway IMMUNOBIOLOGY Qiang Shu IHC Human synovial tissue

Hypoxic preconditioned mesenchymal stem cells ameliorate rat brain injury after cardiopulmonary resuscitation by suppressing neuronal pyroptosis. Feng Chen WB Rat cortical tissue

miR-20b attenuates airway inflammation by regulating TXNIP and NLRP3 inflammasome in ovalbumin-induced asthmatic mice. Yun Sun IF Mouse 1:100 Lung tissue

Induced Neural Stem Cells Suppressed Neuroinflammation by Inhibiting the Microglial Pyroptotic Pathway in Intracerebral Hemorrhage Rats. Xueyun Liang IF Rat,Human 1:200 brain tissue induced neural stem cells (iNSCs)

Targeting the mechanism of IRF3 in sepsis-associated acute kidney injury via the Hippo pathway. Xingui Dai WB Human HK-2 cell

Pristimerin suppresses AIM2 inflammasome by modulating AIM2-PYCARD/ASC stability via selective autophagy to alleviate tendinopathy. Autophagy Xiao Yu WB Mouse Achilles tendon Peritoneal macrophages

A novel drug combination of Tofacitinib and Iguratimod alleviates rheumatoid arthritis and secondary osteoporosis. INTERNATIONAL IMMUNOPHARMACOLOGY Qiang Shu IHC，IF Rat 1:100，1:100 Synovial tissue,bone tissue

Yueliang Yin Ameliorates Endometrial Receptivity in Mice with Embryo Implantation Failure by Reducing Pyroptosis and Activating BDNF/TrkB Pathway. MOLECULAR NUTRITION & FOOD RESEARCH Ting Wang WB Mouse uterus

Piceatannol alleviates liver ischaemia/reperfusion injury by inhibiting TLR4/NF-κB/NLRP3 in hepatic macrophages. EUROPEAN JOURNAL OF PHARMACOLOGY Jian Du IHC，IF，WB Mouse 1:100,1:50,1:1000 liver tissue macrophages

Chlorogenic acid protects against myocardial ischemia–reperfusion injury in mice by inhibiting Lnc Neat1/NLRP3 inflammasome-mediated pyroptosis. Scientific Reports Deqin Lu IHC Mouse cardiomyocytes

Nobiletin Alleviated Epithelial–Mesenchymal Transition of Hepatocytes in Liver Fibrosis Based on Autophagy—Hippo/YAP Pathway MOLECULAR NUTRITION & FOOD RESEARCH Yuting Hao WB Mouse,Human liver tissue L02 cell

Dihydroxyacetone phosphate accumulation leads to podocyte pyroptosis in diabetic kidney disease JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Zongwei Zhang WB Mouse kidney tissue podocytes

NLRP6 deficiency suppresses colorectal cancer liver metastasis growth by modulating M-MDSC-induced immunosuppressive microenvironment BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE Liangzheng Chang IHC Human colorectal cancer (CRC) tissue,liver metastasis tissue

Cathepsin B aggravates atherosclerosis in ApoE-deficient mice by modulating vascular smooth muscle cell pyroptosis through NF-κB / NLRP3 signaling pathway PLoS One Hui Li IHC,WB Mouse 1:200 aorta tissue vascular smooth muscle cells (VSMCs)

Maresin-1 ameliorates hypertensive vascular remodeling through its receptor LGR6 MedComm Zheng Yin WB Mouse,Rat aorta tissue rat aortic smooth muscle cells (RASMCs)

Neutrophil extracellular traps induce pyroptosis of pulmonary microvascular endothelial cells by activating the NLRP3 inflammasome CLINICAL AND EXPERIMENTAL IMMUNOLOGY Zhao Peipei IF,WB Human 1:200 human pulmonary microvascular endothelial cells (HPMECs)

PLD2 deletion ameliorates sepsis-induced cardiomyopathy by suppressing cardiomyocyte pyroptosis via the NLRP3/caspase 1/GSDMD pathway INFLAMMATION RESEARCH Li Jun IHC,WB Rat,Mouse 1:1000 heart tissue H9C2 cardiomyocytes

Exocarpium Citri Grandis ameliorates LPS-induced acute lung injury by suppressing inflammation, NLRP3 inflammasome, and ferroptosis JOURNAL OF ETHNOPHARMACOLOGY Zaibin Xu IF,WB Mouse Lung tissue RAW264.7 cell

Polystyrene nanoplastics induce intestinal and hepatic inflammation through activation of NF-κB/NLRP3 pathways and related gut-liver axis in mice SCIENCE OF THE TOTAL ENVIRONMENT Xuanwei Chen WB Mouse 1:1000 intestinal tissue,liver tissue

Dihydroartemisinin-driven TOM70 inhibition leads to mitochondrial destabilization to induce pyroptosis against lung cancer PHYTOTHERAPY RESEARCH Liu-Gen Li WB,IHC,IF Mouse,Human Lewis cell-Xenograft Lewis cell,A549 cell

Astaxanthin alleviates fibromyalgia pain and depression via NLRP3 inflammasome inhibition BIOMEDICINE & PHARMACOTHERAPY Lin Zhao WB Mouse 1:1000 brain tissue,spinal cord tissue

Sinensetin, a polymethoxyflavone from citrus fruits, ameliorates LPS-induced acute lung injury by suppressing Txnip/NLRP3/Caspase-1/GSDMD signaling-mediated inflammatory responses and pyroptosis Food & Function Zaibin Xu WB Mouse lung tissue RAW264.7 cell

Ethyl pyruvate alleviates NLRP3/Caspase-1/GSDMD-mediated neuronal pyroptosis in neonatal rats with hypoxic–ischemic brain damage INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE Sha Sha WB Rat,Mouse 1:1000 hippocampal tissue HT22 cell

Targeting TANK-binding kinase 1 attenuates painful diabetic neuropathy via inhibiting microglia pyroptosis Cell Communication and Signaling Liao Qinming WB Mouse spinal cord tissue

Thonningianin A ameliorated renal interstitial fibrosis in diabetic nephropathy mice by modulating gut microbiota dysbiosis and repressing inflammation Frontiers in Pharmacology Zhang Shujiao IF,WB Mouse 1:500,1:100 kidney tissue

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