Villin (PT0254) mouse mAb Ready to use

  • Catalog No.:YM6222R
  • Applications:IHC-p;IF(paraffin section)
  • Reactivity:Human
    • Gene Name:
    • VIL1 VIL
    • Protein Name:
    • Villin-1
    • Human Gene Id:
    • 7429
    • Human Swiss Prot No:
    • P09327
    • Immunogen:
    • Synthesized peptide derived from human Villin
    • Specificity:
    • This antibody detects endogenous levels of human Villin. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section
    • Formulation:
    • Liquid in PBS containing, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Monoclonal, Mouse/IgG2b, Kappa
    • Dilution:
    • Ready to use for IHC-p, WB application may demands at least 1:5-20 dilution.
    • Purification:
    • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
    • Storage Stability:
    • 4°C/ 1 years
    • Background:
    • This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008],
    • Function:
    • domain:Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.,function:Ca(2+)-regulated actin-binding protein.,similarity:Belongs to the villin/gelsolin family.,similarity:Contains 1 HP (headpiece) domain.,similarity:Contains 6 gelsolin-like repeats.,subunit:Monomer.,tissue specificity:Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.,
    • Subcellular Location:
    • Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, microvillus. Cell projection, filopodium tip . Cell projection, filopodium . Relocalized in the tip of cellular protrusions and filipodial extensions upon infection with S.flexneri in primary intestinal epithelial cells (IEC) and in the tail-like structures forming the actin comets of S.flexneri. Redistributed to the leading edge of hepatocyte growth factor (HGF)-induced lamellipodia (By similarity). Rapidly redistributed to ruffles and lamellipodia structures in response to autotaxin, lysophosphatidic acid (LPA) and epidermal growth factor (EGF) treatment. .
    • Expression:
    • Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes (at protein level).