Proliferating Cell Nuclear Antigen (PCNA) (ABT-PCNA) mouse mAb Ready to use

  • Catalog No.:YM6090R
  • Applications:IHC-p,WB
  • Reactivity:Human
    • Gene Name:
    • PCNA
    • Protein Name:
    • Proliferating cell nuclear antigen (PCNA) (Cyclin)
    • Human Gene Id:
    • 5111
    • Human Swiss Prot No:
    • P12004
    • Immunogen:
    • Synthesized peptide derived from human Proliferating Cell Nuclear Antigen (PCNA)
    • Specificity:
    • This antibody detects endogenous levels of human Proliferating Cell Nuclear Antigen (PCNA). Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in
    • Formulation:
    • Liquid in PBS containing, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Mouse/IgG1, Kappa
    • Dilution:
    • Ready to use for IHC-p, WB application may demands at least 1:5-20 dilution.
    • Purification:
    • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
    • Storage Stability:
    • 4°C/ 1 years
    • Background:
    • proliferating cell nuclear antigen(PCNA) Homo sapiens The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008],
    • Function:
    • disease:Antibodies are present in sera from patients with systemic lupus erythematosus.,function:This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.,online information:PCNA entry,PTM:Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical poly-ubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases RNF8 and SHPRH, which are required for DNA repair.,similarity:Belongs to the PCNA family.,subunit:Homotrimer. Interacts with KCTD10. Interacts with PPP1R15A (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with POLH, POLK, DNMT1, ERCC5/XPG, FEN1, C
    • Subcellular Location:
    • Nuclear
    • Expression:
    • Bone marrow,Fetal brain cortex,Lung,Placenta,