FLI1 (ABT-FLI1) mouse mAb

    • Catalog No.:YM6057
    • Applications:IHC-p,WB
    • Reactivity:Human
      • Gene Name:
      • FLI1
      • Protein Name:
      • Friend leukemia integration 1 transcription factor (Proto-oncogene Fli-1) (Transcription factor ERGB)
      • Human Gene Id:
      • 2313
      • Human Swiss Prot No:
      • Q01543
      • Immunogen:
      • Synthesized peptide derived from human FLI1
      • Specificity:
      • This antibody detects endogenous levels of human FLI1. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Mouse/IgG1, Kappa
      • Dilution:
      • IHC-p 1:100-500,WB 1:500-2000
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Background:
      • Fli-1 proto-oncogene, ETS transcription factor(FLI1) Homo sapiens This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012],
      • Function:
      • disease:A chromosomal aberration involving FLI1 is a cause of Ewing sarcoma [MIM:133450]. Translocation t(11;22)(q24;q12) with EWS.,function:Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.,miscellaneous:Located on a fragment of chromosome 11 flanked on the centromeric side by the acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation breakpoint and on the telomeric side by the Ewing- and neuroepithelioma-associated t(11;22) (q24;q12) breakpoint.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,similarity:Contains 1 PNT (pointed) domain.,subunit:Can form homodimers or heterodimers with ETV6/TEL1.,
      • Subcellular Location:
      • Nuclear
      • Expression:
      • Blood,Bone marrow,Lymph,Placenta,
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Hemangioendothelioma. 1, Antibody was diluted at 1:200(4°,overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Hemangioendothelioma. 1, Antibody was diluted at 1:200(4°,overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded PNET. 1, Antibody was diluted at 1:200(4°,overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Western blot analysis of FLI1Antibody at 1:1000 dilution.