HSP90β Monoclonal Antibody(M2)

    • Catalog No.:YM3130
    • Applications:WB,IHC-p,IF,
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • HSP90AB1
      • Protein Name:
      • Heat shock protein HSP 90-beta
      • Human Gene Id:
      • 3326
      • Human Swiss Prot No:
      • P08238
      • Mouse Swiss Prot No:
      • P11499
      • Immunogen:
      • Synthetic Peptide of HSP90β
      • Specificity:
      • The antibody detects endogenous HSP90β protein.
      • Formulation:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • Source:
      • Mouse
      • Dilution:
      • WB: 1:1000-3000 IF 1:200 IHC 1:50-300
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • HSP90AB1; HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84
      • MolecularWeight(Da):
      • 83133
      • Observed Band(KD):
      • 90
      • Background:
      • heat shock protein 90 alpha family class B member 1(HSP90AB1) Homo sapiens This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012],
      • Function:
      • function:Molecular chaperone. Has ATPase activity.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the heat shock protein 90 family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Homodimer. Interacts with TP53/p53 (By similarity). Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer.,
      • Subcellular Location:
      • nucleus,nucleoplasm,cytoplasm,mitochondrion,lysosomal membrane,cytosol,cell-cell adherens junction,COP9 signalosome,cell surface,membrane,inclusion body,basolateral plasma membrane,apic
      • Expression:
      • Amygdala,Brain cortex,Colon,Colon carcinoma,Embryon
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,HSP90β Monoclonal Antibody(M2) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,HSP90β Monoclonal Antibody(M2) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,HSP90β Monoclonal Antibody(M2) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunofluorescence analysis of Mouse-spleen tissue. 1,HSP90β Monoclonal Antibody(M2)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunofluorescence analysis of Rat-lung tissue. 1,HSP90β Monoclonal Antibody(M2)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Western blot analysis of 1) Hela, 2) Mouse Brain tissue, 3) Rat Brain tissue, diluted at 1:2000.