• Target：
• IDE

• Fields：
• >>Alzheimer disease

• Gene Name：
• IDE

• Protein Name：
• Insulin-degrading enzyme

• Human Gene Id：
• 3416

• Human Swiss Prot No：
• P14735

• Mouse Swiss Prot No：
• Q9JHR7

• Rat Gene Id：
• 25700

• Rat Swiss Prot No：
• P35559

• Immunogen：
• Synthetic Peptide of IDE

• Specificity：
• The antibody detects endogenous IDE proteins.

• Formulation：
• PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:1000 IF 1:200 IHC 1:50-300

• Purification：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• IDE;Insulin-degrading enzyme;Abeta-degrading protease;Insulin protease;Insulinase;Insulysin

• Observed Band(KD)：
• 118kD

• Background：
• This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been describe

• Function：
• catalytic activity:Degradation of insulin, glucagon and other polypeptides. No action on proteins.,cofactor:Binds 1 zinc ion per subunit.,function:May play a role in the cellular processing of insulin. May be involved in intercellular peptide signaling.,PTM:The N-terminus is blocked.,similarity:Belongs to the peptidase M16 family.,subunit:Homodimer.,

• Subcellular Location：
• Cytoplasm, cytosol . Cell membrane . Secreted . Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.

• Expression：
• Detected in brain and in cerebrospinal fluid (at protein level).

The extracellular matrix enriched with membrane metalloendopeptidase and insulin‐degrading enzyme suppresses the deposition of amyloid‐beta peptide in Alzheimer's disease cell models. Journal of Tissue Engineering and Regenerative Medicine 2019 Aug 28 WB Human,Hamster HFF cell,CHO cell

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