• Target：
• His-Tag

• Gene Name：
• His-Tag

• Protein Name：
• His Tag

• Immunogen：
• Synthetic peptide:HHHHHH conjugated to KLH.

• Specificity：
• The antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins.

• Formulation：
• PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:3000 IP: 1:200 IF 1:1000

• Purification：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Background：
• A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

VAMP4 regulates insulin levels by targeting secretory granules to lysosomes

A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination CoIP Human /HeLa cell，U2OS cell

Facile purification of active recombinant mouse cytosolic carboxypeptidase 6 from Escherichia coli WB Escherichia Coli 1:5000 /

Construction and evaluation of DNA vaccine encoding Ebola virus glycoprotein fused with lysosome-associated membrane protein. ANTIVIRAL RESEARCH Antivir Res. 2021 Sep;193:105141 WB Human 1:3000 HeLa cell

Novel Functional Recombinant Human Follicle-Stimulating Hormone Acquired from Goat Milk. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY J Agr Food Chem. 2021;69(9):2793–2804 Pull down，WB Goat 1:16000 Goat mammary glands GMECs

Cloning, identification of the two cytokine receptor family B subunits CRFB1 and CRFB5 from grass carp (Ctenopharyngodon idella). FISH & SHELLFISH IMMUNOLOGY Fish Shellfish Immun. 2015 Aug;45:211 WB Fish CIK cell

Overexpression of neuregulin 1 in GABAergic interneurons results in reversible cortical disinhibition. Nature Communications Nat Commun. 2021 Jan;12(1):1-13 WB Mouse 1:2000 Prefrontal cotex

HU, Jian-wen, et al. "Antibiotics and ELISA as a Hyphenated Technique for Screening High Production Cell Lines from Transfected HEK293 Cells for the Production of Recombinant Follicle Stimulating Hormone." 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017). Atlantis Press, 2017.

AtHD2D, A Plant-specific Histone Deacetylase Involved in Abscisic Acid Response and Lateral Root Development JOURNAL OF EXPERIMENTAL BOTANY Zhaofen Han Pull down, WB Arabidopsis thaliana

Therapeutic potential of kaempferol on Streptococcus pneumoniae infection

CCP5 and CCP6 retain CP110 and negatively regulate ciliogenesis. Hui-Yuan Wu IP Human 1:2000 HEK293T cell

The wheat VQ motif-containing protein TaVQ4-D positively regulates drought tolerance in transgenic plants. Dong-hong Min Pull down Wheat leaves

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs