• Target：
• Mouse IgG (H&L)

• Formulation：
• 1 mg/ml, liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide

• Source：
• Goat

• Dilution：
• IHC (1:200 - 1:1000), IF (1:200 - 1:1000), FCM (1:100 - 1:1000), Elisa (Use at an assay dependent concentration)

• Purification：
• The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

• Concentration：
• 1mg/mL

• Storage Stability：
• Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezi

• Other Name：
• Goat Anti-Mouse IgG (H&L) - Alexa Fluor 488

• Background：
• Immunoway secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.

Discovery of potent microtubule-destabilizing agents targeting for colchicine site by virtual screening, biological evaluation, and molecular dynamics simulation

UHRF1 plays an oncogenic role in small cell lung cancer MOLECULAR CARCINOGENESIS Jia Hou, Wenyuan Li, Shirong Zhang, Deli Tan, Kejia Lv, Yue Zhu, Yuzhu Hou, Hui Guo, Lili Jiang IF Human DMS114 cell, H1688 cell

Periostin promotes nucleus pulposus cells apoptosis by activating the Wnt/β-catenin signaling pathway IF Rat 1:300 /NPCs

HDAC11 promotes both NLRP3/caspase-1/GSDMD and caspase-3/GSDME pathways causing pyroptosis via ERG in vascular endothelial cells Cell Death Discov. 2022 Mar;8(1):1-11. IF Mouse 1:200 aortic root

Pdcd4 promotes lipid deposition by attenuating PPARα-mediated fatty acid oxidation in hepatocytes Mol Cell Endocrinol. 2022 Apr;545:111562. WB,IF Rat 1:200

Substrate stiffness affects the morphology, proliferation, and radiosensitivity of cervical squamous carcinoma cells. TISSUE & CELL Tissue Cell. 2022 Feb;74:101681 WB，IF Human SiHa cell

PDE9 inhibition promotes proliferation of neural stem cells via cGMP-PKG pathway following oxygen-glucose deprivation/reoxygenation injury in vitro. NEUROCHEMISTRY INTERNATIONAL Neurochem Int. 2020 Feb;133:104630 IF Rat 1:100 NSCs

Hypoxia–CXCL6 axis affects arteriolar niche remodeling in acute myeloid leukemia:. EXPERIMENTAL BIOLOGY AND MEDICINE Exp Biol Med. 2020;246(1):84-96 IF Human 1:500 MSCs , HUAECs

Neuroprotective effect of Hongjing I granules on erectile dysfunction in a rat model of bilateral cavernous nerve injury. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2020 Oct;130:110405 IF Rat 1:500 Penile tissue

Effect of HongJing I in Treating Erectile Function and Regulating RhoA Pathway in a Rat Model of Bilateral Cavernous Nerve Injury. Evidence-based Complementary and Alternative Medicine 2019 Sep 16 IHC Rat 1 : 500 Penile Tissue

Ultrafiltration Extract of Radix Angelica Sinensis and Radix Hedysari Attenuates Risk of Low-Dose X-Ray Radiation-Induced Myocardial Fibrosis In Vitro. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2021;2021:5580828 IF Rat 1: 10000 Cardiac fibroblasts (CFs)

CTI-2 Inhibits Metastasis and Epithelial-Mesenchymal Transition of Breast Cancer Cells by Modulating MAPK Signaling Pathway. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Int J Mol Sci. 2021 Jan;22(22):12229 WB，IF Human MDA-MB-231cell

The Potential Relationship Between HIF-1α and Amino Acid Metabolism After Hypoxic Ischemia and Dual Effects on Neurons. Frontiers in Neuroscience Front Neurosci-Switz. 2021; 15: 676553 IF Pig 1:100 Brain

Involvement of nuclear factor erythroid 2‑related factor 2 in neonatal intestinal interleukin‑17D expression in hyperoxia. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Int J Mol Med. 2020 Oct;46(4):1423-1432 IF Rat 1 : 100 Intestine tissue

Sweroside promotes osteoblastic differentiation and mineralization via interaction of membrane estrogen receptor-α and GPR30 mediated p38 signalling pathway on MC3T3-E1 cells. PHYTOMEDICINE 2019 Dec 07 IF Human MC3T3-E1 cell

ROS-activated MAPK/ERK pathway regulates crosstalk between Nrf2 and Hif-1α to promote IL-17D expression protecting the intestinal epithelial barrier under hyperoxia INTERNATIONAL IMMUNOPHARMACOLOGY Dongyan Liu IF Human NCM460 cell

Losartan attenuates acetic acid enema-induced visceral hypersensitivity by inhibiting the ACE1/Ang II/AT1 receptor axis in enteric glial cells EUROPEAN JOURNAL OF PHARMACOLOGY Fei Dai IF Rat colon tissue enteric glial cells (EGCs)

Coelonin protects against PM2.5-induced macrophage damage via suppressing TLR4/NF-κB/COX-2 signaling pathway and NLRP3 inflammasome activation in vitro ENVIRONMENTAL TOXICOLOGY Zhi-Shan Ding IF Mouse RAW264.7 cell,J774A.1 cell

Dual-functional MOFs-based hybrid microgel advances aqueous lubrication and anti-inflammation JOURNAL OF COLLOID AND INTERFACE SCIENCE Jianxi Liu IF Human human normal chondrocytes (C-28/I2 cells)

Cathepsin B mediates the lysosomal-mitochondrial apoptosis pathway in arsenic-induced microglial cell injury. Baofei Sun IF Mouse 1:200 BV2 cell

Helicobacter pylori promotes gastric intestinal metaplasia through activation of IRF3-mediated kynurenine pathway. Wanfu Xu IF Human,Mouse gastric mucosa tissue BGC823 cell

The novel mechanism of human norovirus induced diarrhea: Activation of PKD2 caused by HuNoVs destroyed AQP3 expression through AP2γ in intestinal epithelial cells LIFE SCIENCES Ling Huang IF Human HT-29 cell

Assessment of osteopontin as an early nephrotoxicity indicator in human renal proximal tubule cells and its application in evaluating lanthanum-induced nephrotoxicity ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Yingsi Chen IF Human induced pluripotent stem cells (iPSCs)

Radiosensitization of Osteosarcoma Cells Using the PARP Inhibitor Olaparib Combined with X-rays or Carbon Ions Journal of Cancer Dong Meng IF Human,Mouse U2OS cell,K7M2 cell

Turning Threat to Therapy: A Nanozyme-Patch in Surgical Bed for Convenient Tumor Vaccination by Sustained In Situ Catalysis Advanced Healthcare Materials Zhaohui Wang

Discovery of D25, a Potent and Selective MNK Inhibitor for Sepsis-Associated Acute Spleen Injury JOURNAL OF MEDICINAL CHEMISTRY Qiang Li IF Mouse RAW 264.7 cell

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs