Phospho NPM (T199) Cell-Based Colorimetric ELISA Kit

  • Catalog No.：KA1377C
  • Applications：ELISA
  • Reactivity：Human;Mouse;Rat
    • Gene Name：
    • NPM1
    • Human Gene Id：
    • 4869
    • Human Swiss Prot No：
    • P06748
    • Mouse Swiss Prot No：
    • Q61937
    • Storage Stability：
    • 2-8°C/6 months
    • Other Name：
    • Nucleophosmin (NPM) (Nucleolar phosphoprotein B23) (Nucleolar protein NO38) (Numatrin)
    • Detection Method：
    • Colorimetric
    • Background：
    • disease:A chromosomal aberration involving NPM1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with MLF1.,disease:A chromosomal aberration involving NPM1 is found in a form of acute promyelocytic leukemia. Translocation t(5;17)(q32;q11) with RARA.,disease:A chromosomal aberration involving NPM1 is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with ALK. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated.,disease:Defects in NPM1 are associated with acute myelogenous leukemia (AML). Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location.,function:Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors TP53/p53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4.,PTM:Acetylated at C-terminal lysine residues, thereby increasing affinity to histones.,PTM:ADP-ribosylated.,PTM:Phosphorylated at Ser-4 by PLK1. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDC2 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphoryled at Ser-70 by NEK2.,PTM:Sumoylated by ARF.,similarity:Belongs to the nucleoplasmin family.,subcellular location:Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus.,subunit:Decamer formed by two pentameric rings associated in a head-to-head fashion. Disulfide-linked dimers under certain conditions. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70 (By similarity). Interacts with NSUN2. Interacts with hepatitis delta virus S-HDAg.,
    • Function：
    • ribosome export from nucleus, ribosomal large subunit export from nucleus, ribosomal small subunit export from nucleus, microtubule cytoskeleton organization, DNA packaging, chromatin organization, chromatin assembly or disassembly, nucleosome assembly, RNA localization, RNA export from nucleus, rRNA export from nucleus, protein complex assembly, cell volume homeostasis, intracellular protein transport, nucleocytoplasmic transport, anti-apoptosis, cytoskeleton organization, microtubule-based process, cell cycle, centrosome cycle, aging, cell aging,protein localization, positive regulation of cell proliferation, negative regulation of cell proliferation, regulation of cell size, positive regulation of biosynthetic process, regulation of cell cycle process, negative regulation of macromolecule metabolic process, positive regulation of organelle organization, negative regulation of organell
    • Subcellular Location：
    • Nucleus, nucleolus . Nucleus, nucleoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.

    Attributes

    • Code：
    • KA1377C
    • Size
    • 96 / well
    • Price$：
    • 470
    • Delivery：
    • 2024-12-08
    Order

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