eIF5A/eIF5A2 Polyclonal Antibody

    • 货号:YT6284
    • 应用:WB;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • eIF5A/eIF5A2
      • 基因名称:
      • EIF5A
      • 蛋白名称:
      • eIF5A/eIF5A2
      • Human Gene Id:
      • 1984
      • 免疫原:
      • Synthesized peptide derived from human eIF5A/eIF5A2
      • 特异性:
      • This antibody detects endogenous levels of human eIF5A/eIF5A2
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500-2000, ELISA(peptide)1:5000-20000
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • Eukaryotic translation initiation factor 5A-1 (eIF-5A-1;eIF-5A1;Eukaryotic initiation factor 5A isoform 1;eIF-5A;Rev-binding factor;eIF-4D)
      • 实测条带:
      • 17kD
      • 功能:
      • function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.,PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).,similarity:Belongs to the eIF-5A family.,subunit:Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4.,tissue specificity:Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).,
      • 细胞定位:
      • Cytoplasm . Nucleus . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions (PubMed:19379712, PubMed:27306458). Nuclear export of hypusinated protein is mediated by XPO4 (PubMed:10944119, PubMed:27306458). .
      • 组织表达:
      • Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).
      • 产品图片
      • Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°over night